Interaction of human leukotriene C4 synthase and microsomal glutathione transferase in vivo
1996 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 229, no 2, 388-395 p.Article in journal (Refereed) Published
Microsomal glutathione transferase (mGT) specifically binds leukotriene C4 synthase in the presence of Mg2+ ion (Söderström et al., Protein Expression and Purification (1995) 6, 352-356). To investigate if this interaction occurs in vivo we screened a human lung cDNA library with a bait vector encoding human mGT in the yeast two-hybrid system. One of the five positive clones obtained encoded leukotriene C4 synthase. This clone was expressed in two heterologous systems. The recombinant protein cross-reacted with a guinea pig antibody raised against a Keyhole limpet hemocyanin coupled synthetic peptide corresponding to amino acids 141-150 of human leukotriene C4 synthase.
Place, publisher, year, edition, pages
Elsevier, 1996. Vol. 229, no 2, 388-395 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-86574DOI: 10.1006/bbrc.1996.1815ISI: A1996VZ79900005PubMedID: 8954909OAI: oai:DiVA.org:liu-86574DiVA: diva2:578855