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Indirect Determination of Mercury Ion by Inhibition of a Glucose Biosensor Based on ZnO Nanorods
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology.
Linköping University, Department of Science and Technology, Physics and Electronics. Linköping University, The Institute of Technology.
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2012 (English)In: Sensors, ISSN 1424-8220, Vol. 12, no 11, 15063-15077 p.Article in journal (Refereed) Published
Abstract [en]

A potentiometric glucose biosensor based on immobilization of glucose oxidase (GOD) on ZnO nanorods (ZnO-NRs) has been developed for the indirect determination of environmental mercury ions. The ZnO-NRs were grown on a gold coated glass substrate by using the low temperature aqueous chemical growth (ACG) approach. Glucose oxidase in conjunction with a chitosan membrane and a glutaraldehyde (GA) were immobilized on the surface of the ZnO-NRs using a simple physical adsorption method and then used as a potentiometric working electrode. The potential response of the biosensor between the working electrode and an Ag/AgCl reference electrode was measured in a 1mM phosphate buffer solution (PBS). The detection limit of the mercury ion sensor was found to be 0.5 nM. The experimental results provide two linear ranges of the inhibition from 0.5 x 10(-6) mM to 0.5 x 10(-4) mM, and from 0.5 x 10(-4) mM to 20 mM of mercury ion for fixed 1 mM of glucose concentration in the solution. The linear range of the inhibition from 10(-3) mM to 6 mM of mercury ion was also acquired for a fixed 10 mM of glucose concentration. The working electrode can be reactivated by more than 70% after inhibition by simply dipping the used electrode in a 10 mM PBS solution for 7 min. The electrodes retained their original enzyme activity by about 90% for more than three weeks. The response to mercury ions was highly sensitive, selective, stable, reproducible, and interference resistant, and exhibits a fast response time. The developed glucose biosensor has a great potential for detection of mercury with several advantages such as being inexpensive, requiring minimum hardware and being suitable for unskilled users.

Place, publisher, year, edition, pages
MDPI , 2012. Vol. 12, no 11, 15063-15077 p.
Keyword [en]
potentiometric inhibition biosensor, mercury, glucose oxidase, ZnO nanorods
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-86654DOI: 10.3390/s121115063ISI: 000311429500043OAI: diva2:580089
Available from: 2012-12-20 Created: 2012-12-20 Last updated: 2015-01-13
In thesis
1. Synthesis of ZnO and transition metals doped ZnO nanostructures, their characterization and sensing applications
Open this publication in new window or tab >>Synthesis of ZnO and transition metals doped ZnO nanostructures, their characterization and sensing applications
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Nanotechnology is a technology of the design and the applications of nanoscale materials with their fundamentally new properties and functions. Nanosensor devices based on nanomaterials provide very fast response, low-cost, long-life time, easy to use for unskilled users, and provide high-efficiency.

1-D ZnO nanostructures materials have great potential applications in various sensing applications. ZnO is a wide band gap (3.37 eV at room temperature) semiconductor materials having large exciton binding energy (60 meV) and excellent chemical stability, electrical, optical, piezoelectric and pyroelectric properties. By doping the transition metals (TM) into ZnO matrix, the properties of ZnO nanostructures can be tuned and its room  temperature ferromagnetic behavior can be enhanced, which provide the TM-doped ZnO nanostructures as promising candidate for optoelectronic, spintronics and high performance sensors based devices. The synthesis of ZnO and TM-doped ZnO nanostructures via the low temperature hydrothermal method is considered a promising technique due to low cost, environmental friendly, simple solution process, diverse 1-D ZnO nanostructures can be achieved, and large scale production on any type of substrate, and their properties can be controlled by the growth parameters. However, to synthesize 1-D ZnO and TM-doped ZnO nanostructures with controlled shape, structure and uniform size distribution on large area substrates with desirable properties, low cost and simple processes are of high interest and it is a big challenge at present.

The main purpose of this dissertation aims to develop new techniques to synthesize 1-D ZnO and (Fe, Mn)-doped ZnO nanostructures via the hydrothermal method, to characterize and to enhance their functional properties for developing sensing devices such as biosensors for clinical diagnoses and environmental monitoring applications, piezoresistive sensors and UV photodetector.

The first part of the dissertation deals with the hydrothermal synthesis of ZnO nanostructures with controlled shape, structure and uniform size distribution under different conditions and their structural characterization. The possible parameters affecting the growth which can alter the morphology, uniformity and properties of the ZnO nanostructures were investigated. Well-aligned ZnO nanorods have been fabricated for high sensitive piezoresistive sensor. The development of creatinine biosensor for clinical diagnoses purpose and the development of glucose biosensor for indirect determination of mercury ions for an inexpensive and unskilled users for environmental monitoring applications with highly sensitive, selective, stable, reproducible, interference resistant, and fast response time have been fabricated based on ZnO nanorods.

The second part of the dissertation presents a new hydrothermal synthesis of (Fe, Mn)-doped-ZnO nanostructures under different preparation conditions, their properties characterization and the fabrication of piezoresistive sensors and UV photodetectors based devices were demonstrated. The solution preparation condition and growth parameters that influences on the morphology, structures and properties of the nanostructures were investigated. The fabrication of Mn-doped-ZnO NRs/PEDOT:PSS Schottky diodes used as high performance piezoresistive sensor and UV photodetector have been studied and Fe-doped ZnO NRs/FTO Schottky diode has also been fabricated for high performance of UV photodetector. Finally, a brief outlook into future challenges and relating new opportunities are presented in the last part of the dissertation.

Place, publisher, year, edition, pages
Linköping: Linköping University Electronic Press, 2014. 74 p.
Linköping Studies in Science and Technology. Dissertations, ISSN 0345-7524 ; 1629
Synthesis ZnO nanostructures, TM-doped ZnO NRs, Hydrothermal method, Biosensors, Piezoresistive sensors, UV photodetectors, Diluted magnetic semiconductors
National Category
Physical Sciences Electrical Engineering, Electronic Engineering, Information Engineering
urn:nbn:se:liu:diva-113237 (URN)10.3384/diss.diva-113237 (DOI)978-91-7519-206-2 (print) (ISBN)
Public defence
2015-01-23, K3, Kåkenhus, Campus Norrköping, Linköpings universitet, Norrköping, 10:00 (English)
Available from: 2015-01-13 Created: 2015-01-13 Last updated: 2015-01-13Bibliographically approved

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