An Fcγ receptor I (CD64)-negative subpopulation of human peripheral blood monocytes is resistant to killing by antigen-activated CD4-positive cytotoxic T cells
1997 (English)In: European Journal of Immunology, ISSN 0014-2980, E-ISSN 1521-4141, Vol. 27, no 9, 2358-2365 p.Article in journal (Refereed) Published
It has been demonstrated that in monocyte/T cell co-cultures activated with recall antigens, cytotoxic T cells were generated which are able to reduce the number of antigen-presenting monocytes. In previous studies we could show that a minor subset of monocytes, the Fc gamma receptor I-negative (CD64(-)) monocytes, exhibits significantly higher antigen-presenting capacity than the main population of monocytes (> 90%) which are Fc gamma receptor I-positive (CD64(+)). Therefore, we addressed the question whether they are also differentially susceptible to T cell-mediated killing. In the present study we demonstrate that the CD64(-) monocyte subset is more resistant to killing by antigen-activated T cells than CD64(+) monocytes, as indicated by a higher viability and recovery of CD64(-) monocytes. This mechanism involves CD95 (Fas) antigen, since monocyte death in co-cultures with antigen-activated T cells could be partially reduced by blocking anti-Fas monoclonal antibodies (mAb). In agreement with this finding, although CD95 antigen was expressed on CD64(+) and CD64(-) monocytes at comparable levels, killing of CD64(-) monocytes by activating anti-Fas mAb was lower than of CD64(+) monocytes.
Place, publisher, year, edition, pages
1997. Vol. 27, no 9, 2358-2365 p.
Apoptosis, CD64-negative monocytes, cytotoxic T cell, dendritic cells, differential expression, enzyme-like protease, fas-mediated apoptosis, flow-cytometry, ifn-alpha, lymphocytes, macrophages, monoclonal-antibodies, presenting cells, recall antigen, resistance to killing
Biochemistry and Molecular Biology Immunology
IdentifiersURN: urn:nbn:se:liu:diva-87072DOI: 10.1002/eji.1830270934ISI: A1997YA32400033OAI: oai:DiVA.org:liu-87072DiVA: diva2:584806