Electrochemical immunosensor with N-doped graphene-modified electrode for label-free detection of the breast cancer biomarker CA 15-3
2013 (English)In: Biosensors and Bioelectronics, ISSN 0956-5663, EISSN 1873-4235, Vol. 43, 25-29 p.Article in journal (Refereed) Published
Highly sensitive and label-free detection of the biomarker carbohydrate antigen 15-3 (CA 15-3) remains a challenge in the diagnosis of breast cancer. Here, a novel electrochemical immunosensor capable of sensitive and label-free detection of CA 15-3 is reported. This unique immunosensor, equipped with a highly conductive graphene (i.e., N-doped graphene sheets)-modified electrode, exhibited significantly increased electron transfer and high sensitivity toward CA 15-3. This novel immunosensor, with a low detection limit of 0.012U/mL, worked well over a broad linear range of 0.1-20U/mL. Unlike conventional immunosensors, which usually involve complicated label processing and time-consuming separations, the use of highly conductive graphene avoids the need for labels and is simple in nature. The strategy developed for this immunosensor provides a promising approach for clinical research and diagnostic applications.
Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 43, 25-29 p.
Electrochemical immunosensor; Graphene sheets; CA15-3; Label-free detection
IdentifiersURN: urn:nbn:se:liu:diva-87298DOI: 10.1016/j.bios.2012.11.037ISI: 000316525300005OAI: oai:DiVA.org:liu-87298DiVA: diva2:588820