New optical method for estimation of protein bound uremic toxins elimination
2012 (English)Conference paper (Refereed)
The aim of this study was to investigate the possibility to determine the amount of removed Indoxyl Sulphate (IS) during dialysis session. An optical method using fluorescence spectra was used.
Eight uremic patients were studied during three dialysis treatments per patient in one week at the Department of Nephrology at Linköping University Hospital. Dialysate samples were taken during each treatment and analyzed at laboratory. IS concentration was estimated using HPLC method, and fluorescence spectra was measured with spectrofluorophotometer. The fluorescence spectral values were transformed into IS concentration using regression model from the total material noted as fluorescence method (F). Removal ratio (RR) was calculated for both. Achieved results were compared regarding mean values and SD and collated with urea reduction ratio (URR) of same dialysis procedures.
Mean RR value (%) for urea was 74.55±8.11. RR for IS estimated by HPLC was 54.01±8.44 % and by F 58.95±10.94 %. The values were not significantly different (p≤0.05).
This study indicates, that it is possible to estimate RR of IS using only fluorescence values of the spent dialysate and achieved parameter can be used for describing the elimination of protein bound uremic toxins during the dialysis procedure.
Place, publisher, year, edition, pages
Springer Berlin/Heidelberg, 2012. 71-74 p.
, IFMBE Proceedings, ISSN 1680-0737 ; 39
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-87609DOI: 10.1007/978-3-642-29305-4_20ISBN: 978-3-642-29304-7 (print)ISBN: 978-3-642-29305-4 (online)OAI: oai:DiVA.org:liu-87609DiVA: diva2:589739
World Congress on Medical Physics and Biomedical Engineering May 26-31, 2012, Beijing, China