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Quantification of total and visceral adipose tissue in fructose-fed rats using water-fat separated single Echo MRI
Uppsala University, Sweden.
Uppsala University, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Work and Environmental Science. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart and Medicine Center, Occupational and Environmental Medicine Center.
The Swedish university of Agricultural Science, Uppsala, Sweden.
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2013 (English)In: Obesity, ISSN 1930-7381, Vol. 21, no 9, E388-E395 p.Article in journal (Refereed) Published
Abstract [en]

Objective: The aim of this study was to setup a rodent model for modest weight gain and an MRI-based quantification of body composition on a clinical 1.5 T MRI system for studies of obesity and environmental factors and their possible association. Design and Methods: Twenty-four 4-week-old female Fischer rats were divided into two groups: one exposed group (n=12) and one control group (n 12). The exposed group was given drinking water containing fructose (5% for 7 weeks, then 20% for 3 weeks). The control group was given tap water. Before sacrifice, whole body MRI was performed to determine volumes of total and visceral adipose tissue and lean tissue. MRI was performed using a clinical 1.5 T system and a chemical shift based technique for separation of water and fat signal from a rapid single echo acquisition. Fat signal fraction was used to separate adipose and lean tissue. Visceral adipose tissue volume was quantified using semiautomated segmentation. After sacrifice, a perirenal fat pad and the liver were dissected and weighed. Plasma proteins were analyzed by Western blot. Results: The weight gain was 5.2% greater in rats exposed to fructose than in controls (P=0.042). Total and visceral adipose tissue volumes were 5.2 cm(3) (P=0.017) and 3.1 cm(3) (P=0.019) greater, respectively, while lean tissue volumes did not differ. The level of triglycerides and apolipoprotein A-I was higher (P=0.034, P=0.005, respectively) in fructose-exposed rats. Conclusions: The setup induced and assessed a modest visceral obesity and hypertriglyceridemia, making it suitable for further studies of a possible association between environmental factors and obesity.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2013. Vol. 21, no 9, E388-E395 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-87626DOI: 10.1002/oby.20229ISI: 000325426600007OAI: diva2:589776
Available from: 2013-01-19 Created: 2013-01-19 Last updated: 2013-11-08

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