liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Expression of the markers BDCA-2 and BDCA-4 and production of interferon-alpha by plasmacytoid dendritic cells in systemic lupus erythematosus
Uppsala University Hospital, Swedenj.
Swedish University of Agriculture Science, Uppsala, Sweden ).
Swedish University of Agriculture Science, Uppsala, Sweden ).
Swedish University of Agriculture Science, Uppsala, Sweden ).
Show others and affiliations
2003 (English)In: Arthritis and Rheumatism, ISSN 0004-3591, E-ISSN 1529-0131, Vol. 48, no 9, 2524-2532 p.Article in journal (Refereed) Published
Abstract [en]


To study the expression of blood dendritic cell antigen 2 (BDCA-2) and BDCA-4 molecules by plasmacytoid dendritic cells (PDCs) in the blood of patients with systemic lupus erythematosus (SLE), and to study PDC production of interferon-α (IFNα) and its inhibition by anti–BDCA-2 and anti–BDCA-4 antibodies.


Peripheral blood mononuclear cells (PBMCs) from SLE patients (SLE PBMCs) and from healthy controls were induced to produce IFNα in vitro by SLE serum containing an endogenous IFNα-inducing factor (SLE-IIF) or by herpes simplex virus type 1 (HSV-1). The frequencies and numbers of BDCA-2–, BDCA-3–, and BDCA-4–expressing cells were analyzed by flow cytometry, and the effects of anti–BDCA-2 and anti–BDCA-4 monoclonal antibodies (mAb) on IFNα production were investigated.


IFNα production by SLE PBMCs induced by SLE-IIF or HSV-1 was decreased compared with that of healthy control PBMCs (P = 0.002 and P = 0.0007, respectively). The proportions of BDCA-2– and BDCA-3–expressing cells in SLE PBMCs were reduced compared with those in PBMCs from healthy controls (P = 0.01 and P = 0.004, respectively). IFNα producers in culture, especially among SLE PBMCs, displayed reduced BDCA-2 expression and constituted only a minority of the BDCA-2–positive cells, at least in healthy control PBMCs (median 18%). IFNα production by both SLE and healthy control PBMCs stimulated by SLE-IIF or HSV-1 was markedly reduced by anti–BDCA-2 mAb (median 81–98% inhibition). Anti–BDCA-4 mAb only partially inhibited SLE-IIF–induced IFNα production.


SLE patients had a reduced number of BDCA-2–expressing PDCs, also termed natural IFNα-producing cells, and their IFNα production could be inhibited by anti–BDCA-2/4 mAb. Such mAb may be a therapeutic option for inhibiting the ongoing IFNα production in SLE patients.

Place, publisher, year, edition, pages
John Wiley & Sons, 2003. Vol. 48, no 9, 2524-2532 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-87945DOI: 10.1002/art.11225ISI: 000185431900017PubMedID: 13130472OAI: diva2:601200
Available from: 2013-01-28 Created: 2013-01-28 Last updated: 2013-02-12

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Magnusson, Mattias
In the same journal
Arthritis and Rheumatism
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 109 hits
ReferencesLink to record
Permanent link

Direct link