Functional gene transfer of HIV DNA by an HIV receptor-independent mechanism
1999 (English)In: Journal of Immunology, ISSN 0022-1767, E-ISSN 1550-6606, Vol. 163, no 2, 736-742 p.Article in journal (Refereed) Published
HIV-1 enters target cells mainly via binding to CD4 and its coreceptors. The presence of HIV-1 in CD4(-) cells suggests, however, that there exist other mechanisms for viral entry, Here it is reported that HIV-1 DNA may be transferred from one cell to another by uptake of apoptotic bodies in a CD4-independent way. This was investigated by coculturing CD4(-), chemokine receptor CCR5(-) and CXCR4(-) human fetal fibroblasts with apoptotic HIV-1-infected HuT78 cells or apoptotic PBMC isolated from HIV-1-infected patients. After 2 wk of coculture, fibroblasts contained HIV-1 DNA and expressed HIV-1 proteins p24 and gp120, Transfer of HIV-1 DNA was verified by coculturing fibroblasts with apoptotic bodies derived from cells infected with a defective HIV-1 virus. These cells contain one integrated copy of a reverse transcriptase (RT)-negative HIV-1 strain (8E5/LAV RT- cells) and consequently cannot produce free virus, Intracellular HIV-1 gag DNA was detected in both fibroblasts and dendritic cells after coculture with apoptotic 8E5/LAV RT- cells. Transfer of viral DNA after uptake of apoptotic bodies mag explain HIV-1 infection of CD4(-) cells in vivo and furthermore may be relevant for Ag presentation.
Place, publisher, year, edition, pages
American Association of Immunologists , 1999. Vol. 163, no 2, 736-742 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-88116ISI: 000081360800027PubMedID: 10395665OAI: oai:DiVA.org:liu-88116DiVA: diva2:601813