Determination of urea with an ammonia gas-sensitive semiconductor device in combination with urease
1984 (English)In: Analytica Chimica Acta, ISSN 0003-2670, E-ISSN 1873-4324, Vol. 163, 143-149 p.Article in journal (Refereed) Published
An ammonia gas-sensitive Ir/Pd MOS capacitor is used for urea determinations with the aid of urease in two different systems. One combination utilizes a reaction column with immobilized urease in a flow-injection system. The lower limit of urea detection for 150-μl samples was 0.2 μM. Urea in whole blood and blood serum was determined after a 500-fold dilution, and 15 samples per hour could be assayed. The relative standard deviation was 4.6% (n=10). Recovery tests were satisfactory. Values obtained for urea in serum correlated well with those from a spectrophotometric method. The other combination is based on a small flow cell with free urease enclosed between a dialysis membrane and a gas-permeable membrane. Urea was determined in the concentration range 0.01–50 mM. The enzyme probe could be used for up to four days without changes of behaviour.
Place, publisher, year, edition, pages
Elsevier, 1984. Vol. 163, 143-149 p.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-88168DOI: 10.1016/S0003-2670(00)81503-2ISI: A1984TY39000017OAI: oai:DiVA.org:liu-88168DiVA: diva2:601823