Localization of cholesterol, amyloid and glia in Alzheimers disease transgenic mouse brain tissue using time-of-flight secondary ion mass spectrometry (ToF-SIMS) and immunofluorescence imaging
2013 (English)In: Acta Neuropathologica, ISSN 0001-6322, E-ISSN 1432-0533, Vol. 125, no 1, 145-157 p.Article in journal (Refereed) Published
The spatial distributions of lipids, amyloid-beta deposits, markers of neurons and glial cells were imaged, at submicrometer lateral resolution, in brain structures of a mouse model of Alzheimers disease using a new methodology that combines time-of-flight secondary ion mass spectrometry (ToF-SIMS) and confocal fluorescence microscopy. The technology, which enabled us to simultaneously image the lipid and glial cell distributions in Tg2576 mouse brain structures, revealed micrometer-sized cholesterol accumulations in hippocampal regions undergoing amyloid-beta deposition. Such cholesterol granules were either associated with individual amyloid deposits or spread over entire regions undergoing amyloidogenesis. Subsequent immunohistochemical analysis of the same brain regions showed increased microglial and astrocytic immunoreactivity associated with the amyloid deposits, as expected from previous studies, but did not reveal any particular astrocytic or microglial feature correlated with cholesterol granulation. However, dystrophic neurites as well as presynaptic vesicles presented a distribution similar to that of cholesterol granules in regions undergoing amyloid-beta accumulation, thus indicating that these neuronal endpoints may retain cholesterol in areas with lesions. In conclusion, the present study provides evidence for an altered cholesterol distribution near amyloid deposits that would have been missed by several other lipid analysis methods, and opens for the possibility to study in detail the putative liaison between lipid environment and protein structure and function in Alzheimers disease.
Place, publisher, year, edition, pages
Springer Verlag (Germany) , 2013. Vol. 125, no 1, 145-157 p.
ToF-SIMS, Imaging, Amyloid-beta, Alzheimers disease, Cholesterol, Glia, Brain
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-87948DOI: 10.1007/s00401-012-1046-9ISI: 000313002300011OAI: oai:DiVA.org:liu-87948DiVA: diva2:601828
Funding Agencies|EU||FP7 HEALTH Project LUPAS||Fundacio La Marato de TV3||Swedish Research Council (VR)||Swedish Governmental Agency for Innovation Systems (VINNOVA)||Stiftelsen for Gamla Tjanarinnor||Alzheimerfonden||ISC3|PI10/00283|Stockholm County Council||Swedish Foundation for Strategic Research||Knut and Alice Wallenberg foundation||2013-01-302013-01-282015-05-28