liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Disialo–trisialo bridging of transferrin is due to increased branching and fucosylation of the carbohydrate moiety
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Diagnostics, Department of Clinical Chemistry.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Centre for Diagnostics, Department of Clinical Chemistry.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
2012 (English)In: Clinica Chimica Acta, ISSN 0009-8981, E-ISSN 1873-3492, Vol. 414, 58-64 p.Article in journal (Refereed) Published
Abstract [en]

Background

Carbohydrate deficient transferrin (CDT) is used for detection of alcohol abuse and follow-up. High performance liquid chromatography (HPLC) of transferrin glycoforms is highly specific for identification of alcohol abuse, but unresolved disialo- and trisialotransferrin glycoforms sometimes makes interpretation difficult. The cause of this phenomenon is unknown, cannot be explained by genetic variants of transferrin, but seems to be associated with liver disease.

Methods

Nineteen serum samples showing di–tri bridging when analyzed by HPLC were collected. Transferrin was purified by affinity chromatography, and N-linked oligosaccharides were released enzymatically. The N-glycans were further analyzed by high performance anion-exchange chromatography with pulsed amperometric detection and MALDI-TOF mass spectrometry.

Results

The HPLC-analysis showed three different types of glycoform patterns. The N-glycans from fifteen samples showed patterns with increased number of triantennary structures containing one or two fucose residues. One sample contained an increased amount of triantennary glycans without fucose. Three samples showed a glycosylation pattern similar to normal transferrin.

Conclusions

The di–tri bridging phenomenon was associated with alterations in transferrin glycosylation in the majority of cases. Transferrin contained a higher extent of triantennary and often fucosylated N-linked oligosaccharides. These results may be important in future diagnostic approaches to liver diseases.

Place, publisher, year, edition, pages
Elsevier , 2012. Vol. 414, 58-64 p.
Keyword [en]
Transferrin, CDT, Di-tri bridging, Glycosylation, Liver disease
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-88368DOI: 10.1016/j.cca.2012.07.026ISI: 000312685400014OAI: oai:DiVA.org:liu-88368DiVA: diva2:602875
Note

Funding Agencies|Medical Research Council of Southeast Sweden||

Available from: 2013-04-03 Created: 2013-02-04 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

fulltext(988 kB)782 downloads
File information
File name FULLTEXT01.pdfFile size 988 kBChecksum SHA-512
d04049c7c7eb8d1bbd59bba98769bed9f15cab99edf954ae9c3839059a5b9eb82c4a76946a8ec10487e91cfe042b3a83ac47d74df28ade31b115d8b16bcb0629
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Landberg, EvaÅström, EvaKågedal, BertilPåhlsson, Peter

Search in DiVA

By author/editor
Landberg, EvaÅström, EvaKågedal, BertilPåhlsson, Peter
By organisation
Clinical ChemistryFaculty of Health SciencesDepartment of Clinical ChemistryCell Biology
In the same journal
Clinica Chimica Acta
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 782 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 181 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf