Total internal reflection ellipsometry: thickness sensitivity in the pm-range for protein adsorption
(English)Manuscript (preprint) (Other academic)
Total internal reflection ellipsometry (TIRE) is used to study adsorption of human serum albumin and fibrinogen on thin gold films. TIRE shows very high sensitivity for protein mono-layers adsorbed on metal surfaces when surface plasmon resonance effects are ut ilised. The measured data, expressed in ellipsometric angles ψ and Δ are of several orders of magnitude larger in comparison to those from similar experiments performed with traditional ellipsometry. TIRE opens a new path for precise studies of organic layers adsorbed on metal surfaces, with a potential for resolving the adsorbed layer micro-structure.
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-89558OAI: oai:DiVA.org:liu-89558DiVA: diva2:608194