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Osteogenically-induced human dermal fibroblasts as a tool to regenerate bone
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Cell Biology. Linköping University, Faculty of Health Sciences.
Haukeland Hospital, Norway .
Haukeland Hospital, Norway .
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2013 (English)In: Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery, ISSN 2000-656X, E-ISSN 2000-6764, Vol. 47, no 1, 8-13 p.Article in journal (Refereed) Published
Abstract [en]

Engineering of bone tissue could help to overcome the need for extensive reconstruction and associated donor site morbidity, and it has been proposed that osteogenic biomaterials, which are scaffolds that contain osteocompetent cells, could be used to fill large bone defects. This study investigated the potential of osteogenically-induced human dermal fibroblasts cultured on gelatin microcarriers combined with platelet-rich plasma in a model of a femoral defect in athymic rats. Defects were transplanted with one of the following six combinations: 1 = sodium chloride, 2 = platelet-rich plasma, 3 = microcarriers + platelet-rich plasma, 4 = human dermal fibroblasts on microcarriers + platelet-rich plasma, 5 = human osteoblasts on microcarriers + platelet-rich plasma, and 6 = osteogenically induced human dermal fibroblasts on microcarriers + platelet-rich plasma. The femoral defects were assessed 4 weeks postoperatively with computed tomography (CT), routine histological staining, fluorescence in situ hybridisation, and polyclonal antibodies directed towards osteocalcin and osteonectin. Radiographs of all groups taken 4 weeks postoperatively showed unhealed defects. Femoral defects transplanted with osteogenically-induced human dermal fibroblasts on microcarriers (group 6) contained dense clusters of cells with large quantities of extracellular matrix. These clusters were exclusive to this group and stained strongly for osteocalcin and osteonectin. Fluorescence in situ hybridisation showed viable human cells in femoral defects that had been transplanted with microcarriers seeded with cells, which confirmed the survival of implanted cells. In conclusion, osteogenically-induced human dermal fibroblasts survived in this new niche, and bone-like structures were apparent in the defects.

Place, publisher, year, edition, pages
Informa Healthcare , 2013. Vol. 47, no 1, 8-13 p.
Keyword [en]
Tissue engineering, stem cells, human dermal fibroblasts, differentiation, osteogenic induction, formation of bone
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-89513DOI: 10.3109/2000656X.2012.731411ISI: 000313681700002OAI: oai:DiVA.org:liu-89513DiVA: diva2:608377
Available from: 2013-02-27 Created: 2013-02-26 Last updated: 2017-12-06

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Sommar, PehrJunker, JohanHansson, ThomasJohnson, HansKratz, Gunnar

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Sommar, PehrJunker, JohanHansson, ThomasJohnson, HansKratz, Gunnar
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Department of Clinical and Experimental MedicineFaculty of Health SciencesCell BiologySurgeryDepartment of Plastic Surgery, Hand surgery UHLPlastic Surgery, Hand Surgery and Burns
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Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
Medical and Health Sciences

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