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Multicolor Fluorescent Semiconducting Polymer Dots with Narrow Emissions and High Brightness
University of Washington, WA USA .
Jilin University, Peoples R China .
University of Washington, WA USA .
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, Faculty of Science & Engineering.
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2013 (English)In: ACS Nano, ISSN 1936-0851, E-ISSN 1936-086X, Vol. 7, no 1, 376-384 p.Article in journal (Refereed) Published
Abstract [en]

Fluorescent semiconducting polymer dots (Pdots) have attracted great interest because of their superior characteristics as fluorescent probes, such as high fluorescence brightness, fast radiative rates, and excellent photostability. However, currently available Pdots generally exhibit broad emission spectra, which significantly limit their usefulness in many biological applications Involving multiplex detections. Here, we describe the design and development of multicolor narrow emissive Pdots based on different boron dipyrromethene (BODIPY) units. BODIPY-containing semiconducting polymers emitting at multiple wavelengths were synthesized and used as precursors for preparing the Pdots, where intraparticle energy transfer led to highly bright, narrow emissions. The emission full width at half-maximum of the resulting Pdots varies from 40 to 55 nm, which is 15-2 times narrower than those of conventional semiconducting polymer dots. BODIPY 520 Pdots were about an order of magnitude brighter than commercial Qdot 525 under identical laser excitation conditions. Fluorescence imaging and flow cytometry experiments indicate that the narrow emissions from these bright Pdots are promising for multiplexed biological detections.

Place, publisher, year, edition, pages
American Chemical Society , 2013. Vol. 7, no 1, 376-384 p.
Keyword [en]
polymer dots, fluorescence, semiconducting polymer, bioimaging, narrow emission
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-89814DOI: 10.1021/nn304376zISI: 000314082800041OAI: diva2:609777

Funding Agencies|National Institutes of Health|CA147831NS062725|University of Washington||

Available from: 2013-03-07 Created: 2013-03-07 Last updated: 2015-05-29

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Zhang, XuanjunUvdal, Kajsa
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