liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Expression of Integrin alpha 2 Receptor in Human Cord Blood CD34+CD38-CD90+Stem Cells Engrafting Long-Term in NOD/SCID-IL2R gamma(c)null Mice
Lund University, Sweden .
Linköping University, Department of Clinical and Experimental Medicine, Experimental Hematology. Linköping University, Faculty of Health Sciences.
Lund University, Sweden .
BC Cancer Agency, Canada .
Show others and affiliations
2013 (English)In: Stem Cells, ISSN 1066-5099, Vol. 31, no 2, 360-371 p.Article in journal (Refereed) Published
Abstract [en]

Human hematopoietic stem cells reside in the CD34+CD38-CD90+ population in cord blood and bone marrow. However, this cell fraction is heterogeneous, and the phenotype of the rare primitive stem cells remains poorly defined. We here report that primitive cord blood CD34+CD38-CD90+ stem cells, with the ability to reconstitute NOD/SCID-IL2R gamma(c)null (NSG) mice long-term, at 24 weeks after transplantation, can be prospectively isolated at an increased purity by using integrin alpha 2 receptor as an additional stem cell marker. Using a limiting dilution transplantation assay, we found a highly significant enrichment of multilineage reconstituting stem cells in the CD34+CD38-CD90+ cell fraction expressing the integrin alpha 2 receptor, with a frequency of 1/29 cells, as compared to a frequency of 1/157 in the corresponding integrin alpha 2- cells. In line with this, long-term reconstituting stem cells within the cord blood CD34+CD38- cell population were significantly enriched in the integrin alpha 2+ fraction, while stem cells and progenitors reconstituting short-term, at 8-12 weeks, were heterogeneous in integrin alpha 2 expression. Global gene expression profiling revealed that the lineage-marker negative (Lin-) CD34+CD38-CD90+CD45RA- integrin alpha 2+ cell population was molecularly distinct from the integrin alpha 2- cell population and the more mature Lin-CD34+CD38-CD90-CD45RA- cell population. Our findings identify integrin alpha 2 as a novel stem cell marker, which improves prospective isolation of the primitive human hematopoietic stem cells within the CD34+CD38-CD90+ cell population for experimental and therapeutic stem cell applications. STEM CELLS 2013;31:360-371

Place, publisher, year, edition, pages
AlphaMed Press , 2013. Vol. 31, no 2, 360-371 p.
Keyword [en]
Hematopoietic stem cells, Fetal blood, Integrin alpha2, Human
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-90073DOI: 10.1002/stem.1282ISI: 000314873000015OAI: diva2:612427

Funding Agencies|Swedish Cancer Society|CAN 2010/590CAN 2009/1589|Swedish Research Council|2008-19616-59131-19K2008-77PK-20879-01-2|Medical Faculty, Lund University||Skane University Hospital||Gunnar Nilsson Foundation||John Persson Foundation||Gunnel Bjork Foundation||Georg Danielsson Foundation||Karolinska Institute Wallenberg Institute for Regenerative Medicine||

Available from: 2013-03-21 Created: 2013-03-19 Last updated: 2013-03-21

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Sigvardsson, MikaelQian, Hong
By organisation
Experimental HematologyFaculty of Health Sciences
In the same journal
Stem Cells
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 124 hits
ReferencesLink to record
Permanent link

Direct link