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Importance of having low-density functional groups for generating high-performance semiconducting polymer dots
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
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2012 (English)In: ACS Nano, ISSN 1936-0851, E-ISSN 1936-086X, Vol. 6, no 6, 5429-5439 p.Article in journal (Refereed) Published
Abstract [en]

Semiconducting polymers with low-density side-chain carboxylic acid groups were synthesized to form stable, functionalized, and highly fluorescent polymer dots (Pdots). The influence of the molar fraction of hydrophilic side-chains on Pdot properties and performance was systematically investigated. Our results show that the density of side-chain carboxylic acid groups significantly affects Pdot stability, internal structure, fluorescence brightness, and nonspecific binding in cellular labeling. Fluorescence spectroscopy, single-particle imaging, and a dye-doping method were employed to investigate the fluorescence brightness and the internal structure of the Pdots. The results of these experiments indicate that semiconducting polymers with low density of side-chain functional groups can form stable, compact, and highly bright Pdots as compared to those with high density of hydrophilic side-chains. The functionalized polymer dots were conjugated to streptavidin (SA) by carbodiimide-catalyzed coupling and the Pdot-SA probes effectively and specifically labeled the cancer cell-surface marker Her2 in human breast cancer cells. The carboxylate-functionalized polymer could also be covalently modified with small functional molecules to generate Pdot probes for click chemistry-based bio-orthogonal labeling. This study presents a promising approach for further developing functional Pdot probes for biological applications.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2012. Vol. 6, no 6, 5429-5439 p.
Keyword [en]
Semiconducting polymer dots, specific cellular targeting
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-90562DOI: 10.1021/nn301308wOAI: oai:DiVA.org:liu-90562DiVA: diva2:613820
Available from: 2013-04-02 Created: 2013-04-02 Last updated: 2017-12-06

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Zhang, Xuanjun

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CiteExportLink to record
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  • apa
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