Ratiometric temperature sensing with semiconducting polymer dots
2011 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 133, no 21, 8146-8149 p.Article in journal (Refereed) Published
This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye—Rhodamine B (RhB), whose emission intensity decreases with increasing temperature—within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2011. Vol. 133, no 21, 8146-8149 p.
Natural Sciences Chemical Sciences
IdentifiersURN: urn:nbn:se:liu:diva-90567DOI: 10.1021/ja202945gOAI: oai:DiVA.org:liu-90567DiVA: diva2:613840