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Ratiometric temperature sensing with semiconducting polymer dots
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
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2011 (English)In: Journal of the American Chemical Society, ISSN 0002-7863, E-ISSN 1520-5126, Vol. 133, no 21, 8146-8149 p.Article in journal (Refereed) Published
Abstract [en]

This communication describes ultrabright single-nanoparticle ratiometric temperature sensors based on semiconducting polymer dots (Pdots). We attached the temperature sensitive dye—Rhodamine B (RhB), whose emission intensity decreases with increasing temperature—within the matrix of Pdots. The as-prepared Pdot-RhB nanoparticle showed excellent temperature sensitivity and high brightness because it took advantage of the light harvesting and amplified energy transfer capability of Pdots. More importantly, the Pdot-RhB nanoparticle showed ratiometric temperature sensing under a single wavelength excitation and has a linear temperature sensing range that matches well with the physiologically relevant temperatures. We employed Pdot-RhB for measuring intracellular temperatures in a live-cell imaging mode. The exceptional brightness of Pdot-RhB allows this nanoscale temperature sensor to be used also as a fluorescent probe for cellular imaging.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2011. Vol. 133, no 21, 8146-8149 p.
National Category
Natural Sciences Chemical Sciences
Identifiers
URN: urn:nbn:se:liu:diva-90567DOI: 10.1021/ja202945gOAI: oai:DiVA.org:liu-90567DiVA: diva2:613840
Available from: 2013-04-02 Created: 2013-04-02 Last updated: 2017-12-06

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Zhang, Xuanjun

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