liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
High-intensity near-IR fluorescence in semiconducting polymer dots achieved by cascade FRET strategy
Linköping University, Department of Physics, Chemistry and Biology, Molecular Surface Physics and Nano Science. Linköping University, The Institute of Technology.
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
Department of Chemistry, University of Washington, Seattle, USA.
Show others and affiliations
2013 (English)In: Chemical Science, ISSN 2041-6520, E-ISSN 2041-6539, Vol. 4, no 5, 2143-2151 p.Article in journal (Refereed) Published
Abstract [en]

Near-IR (NIR) emitting semiconducting polymer dots (Pdots) with ultrabright fluorescence have been prepared for specific cellular targeting. A series of π-conjugated polymers were synthesized to form water dispersible multicomponent Pdots by an ultrasonication-assisted co-precipitation method. By optimizing cascade energy transfer in Pdots, high-intensity NIR fluorescence (Φ = 0.32) with tunable excitations, large absorption–emission separation (up to 330 nm), and narrow emission bands (FWHM = 44 nm) have been achieved. Single-particle fluorescence imaging show that the as-prepared NIR Pdots were more than three times brighter than the commercially available Qdot705 with comparable sizes under identical conditions of excitation and detection. Because of the covalent introduction of carboxylic acid groups into polymer side chains, the bioconjugation between NIR-emitting Pdots and streptavidins can be readily completed via these functional groups on the surface of Pdots. Furthermore, through flow cytometry and confocal fluorescence microscopy the NIR-emitting Pdot–streptavidin conjugates proved that they could effectively label EpCAM receptors on the surface of MCF-7 cells, via specific binding between streptavidin and biotin.

Place, publisher, year, edition, pages
RSC Publishing, 2013. Vol. 4, no 5, 2143-2151 p.
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:liu:diva-90660DOI: 10.1039/c3sc50222hISI: 000316966500028OAI: oai:DiVA.org:liu-90660DiVA: diva2:614071
Available from: 2013-04-03 Created: 2013-04-03 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

fulltext(2533 kB)850 downloads
File information
File name FULLTEXT01.pdfFile size 2533 kBChecksum SHA-512
1083dbf2506cb9b5f125930aad04661d8b1029991d662bd9ff4794a23432d47b67225090790883984bfca2b99e65c4199f8e79be36eda1ec4bc65e9c3efa9703
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Zhang, XuanjunUvdal, Kajsa

Search in DiVA

By author/editor
Zhang, XuanjunUvdal, Kajsa
By organisation
Molecular Surface Physics and Nano ScienceThe Institute of Technology
In the same journal
Chemical Science
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 850 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 209 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf