liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
A liquid chromatography and tandem mass spectrometry method for the determination of potential biomarkers of cardiovascular disease
Silesian Technical University, Poland .
Silesian Technical University, Poland .
Regional Specialist Hospital, Poland .
Östergötlands Läns Landsting, Heart and Medicine Centre, Department of Clinical Physiology UHL.
2013 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 919, 20-29 p.Article in journal (Refereed) Published
Abstract [en]

A simple, accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantitation of alpha-ketoglutaric acid (alpha-KG), L-carnitine (L-CAR) and acetyl-L-carnitine (acetyl-L-CAR) in human urine as potential biomarkers of cardiovascular disease. The separation was performed using an isocratic elution of 0.1% formic acid in water and acetonitrile (97:3, v/v) on an Acclaim 120 C8 column (150 mm x 4.6 mm, 3.0 mu m). The flow rate of the mobile phase was 1.2 mL/min and the total assay run time was 3 min. Detection was performed on a triple-quadrupole mass spectrometer in selected reaction monitoring (SRM) mode via an electrospray ionization (ESI) source in positive and negative ion modes. This method covered a linearity range of 0.1-500 ng/mL for L-CAR and acetyl-L-CAR and 1-1000 ng/mL for alpha-KG with lower limits of quantification (LLOQ) of 0.08 ng/mL for L-CAR, 0.04 ng/mL for acetyl-L-CAR and 0.8 ng/mL for alpha-KG. The intra-day and inter-day precision and accuracy of the quality control samples exhibited relative standard deviations of less than 5.54% and relative error values from -5.95% to 3.11%. Analyte stability was evaluated under various sample preparation, analysis and storage conditions and varied from -9.89% to -0.47%. A two-step solid-phase extraction (SPE) procedure using silica gel and quaternary amine cartridges was used for urine sample cleanup. The average recoveries for all analyzed compounds were better than 86.64% at three concentrations. The method was successfully applied for the quantitation of alpha-KG, L-CAR and acetyl-L-CAR in human urine samples.

Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 919, 20-29 p.
Keyword [en]
LC-MS/MS, Biomarkers, alpha-Ketoglutaric acid, L-Carnitine, Acetyl-L-carnitine
National Category
Engineering and Technology
URN: urn:nbn:se:liu:diva-90758DOI: 10.1016/j.jchromb.2012.12.015ISI: 000316043300003OAI: diva2:617212

Funding Agencies|National Science Center (Cracow, Poland)|N N204 355840|

Available from: 2013-04-22 Created: 2013-04-05 Last updated: 2013-04-29

Open Access in DiVA

No full text

Other links

Publisher's full text
By organisation
Department of Clinical Physiology UHL
In the same journal
Journal of chromatography. B
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 63 hits
ReferencesLink to record
Permanent link

Direct link