Luminescent Conjugated Oligothiophenes for Sensitive Fluorescent Assignment of Protein Inclusion Bodies
2013 (English)In: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 14, no 5, 607-616 p.Article in journal (Refereed) Published
Small hydrophobic ligands identifying intracellular protein deposits are of great interest, as protein inclusion bodies are the pathological hallmark of several degenerative diseases. Here we report that fluorescent amyloid ligands, termed luminescent conjugated oligothiophenes (LCOs), rapidly and with high sensitivity detect protein inclusion bodies in skeletal muscle tissue from patients with sporadic inclusion body myositis (s-IBM). LCOs having a conjugated backbone of at least five thiophene units emitted strong fluorescence upon binding, and showed co-localization with proteins reported to accumulate in s-IBM protein inclusion bodies. Compared with conventional amyloid ligands, LCOs identified a larger fraction of immunopositive inclusion bodies. When the conjugated thiophene backbone was extended with terminal carboxyl groups, the LCO revealed striking spectral differences between distinct protein inclusion bodies. We conclude that 1) LCOs are sensitive, rapid and powerful tools for identifying protein inclusion bodies and 2) LCOs identify a wider range of protein inclusion bodies than conventional amyloid ligands.
Place, publisher, year, edition, pages
Wiley-VCH Verlag Berlin , 2013. Vol. 14, no 5, 607-616 p.
amyloid beta-peptides, biosensors, fluorescent probes, luminescent conjugated oligothiophene, protein inclusion body
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-91341DOI: 10.1002/cbic.201200731ISI: 000316285600010OAI: oai:DiVA.org:liu-91341DiVA: diva2:617420
Funding Agencies|Swedish Foundation for Strategic Research||European Research Council (ERC)||EU||2013-04-232013-04-222014-04-08Bibliographically approved