SPION primes THP1 derived M2 macrophages towards M1-like macrophages
2013 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 441, no 4, 737-742 p.Article in journal (Refereed) Published
Potentially, cellular iron regulates functional plasticity in macrophages yet; interaction of functionally polarized macrophages with iron-oxide nanoparticles has never been studied. We found that monocyte differentiation alters cellular ferritin and cathepsin L levels and induces functional polarization in macrophages. Iron in super paramagnetic iron-oxide nanoparticle (SPION) induces a phenotypic shift in THP1 derived M2 macrophages towards a high CD86+ macrophage subtype. This phenotypic shift was accompanied by up-regulated intracellular levels of ferritin and cathepsin L in M2 macrophages, which we found as a characteristic hallmark of M1 macrophages. Atherogenic oxysterols reduce phagocytic activity in both macrophage subtypes and thus these cells may escape detection by ironoxide nanoparticles (INPs) in-vivo.
Place, publisher, year, edition, pages
Elsevier, 2013. Vol. 441, no 4, 737-742 p.
Cathepsin L; Ferritin; Iron-oxide nanoparticles; M1 and M2 macrophages; Oxysterols
IdentifiersURN: urn:nbn:se:liu:diva-91999DOI: 10.1016/j.bbrc.2013.10.115ISI: 000328434800008OAI: oai:DiVA.org:liu-91999DiVA: diva2:619955