liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Diffusion controlled and temperature stable microcapsule reaction compartments for high throughput microcapsule-PCR
National University of Singapore.ORCID iD: 0000-0003-3274-6029
National University of Singapore.
National University of Singapore.
2008 (English)In: Advanced Functional Materials, ISSN 1616-301X, E-ISSN 1616-3028, Vol. 18, no 19, 2930-2937 p.Article in journal (Refereed) Published
Abstract [en]

A novel approach to perform a high number of individual polymerase chain reactions (PCR) in microcapsule reaction compartments, termed “Microcapsule-PCR” was developed. Temperature stable microcapsules with a selective permeable capsule wall were constructed by matrix-assisted layer-by-layer (LbL) Encapsulation technique. During the PCR, small molecular weight building blocks – nucleotides (dNTPs) were supplied externally and diffuse through the permeable capsule wall into the interior, while the resulted high molecular weight PCR products were accumulated within the microcapsule. Microcapsules (∼110.8 µm average diameter) filled with a PCR reaction mixture were constructed by an emulsion technique having a 2% agarose core and a capsule formed by LbL coating with poly(allylamine-hydrochloride) and poly(4-styrene-sulfonate). An encapsulation efficiency of 47% (measured for primer-FITC (22 bases)) and 98% PCR efficiency was achieved. Microcapsules formed by eight layers of polyelectrolyte and subjected to PCR cycling (up to 95 °C) demonstrated good temperature stability without any significantly changes in DNA retention yield and microcapsule morphology. A multiplex Microcapsule-PCR experiment demonstrated that microcapsules are individual compartment and do not exchange templates or primers between microcapsules during PCR cycling.

Place, publisher, year, edition, pages
John Wiley & Sons, 2008. Vol. 18, no 19, 2930-2937 p.
Keyword [en]
layer-by-layer assembly; microencapsulation; microreactors; polymerization; polyelectrolytes
National Category
Materials Chemistry
URN: urn:nbn:se:liu:diva-92065DOI: 10.1002/adfm.200800388OAI: diva2:620049
Available from: 2013-05-07 Created: 2013-05-07 Last updated: 2015-03-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Mak, Wing Cheung
In the same journal
Advanced Functional Materials
Materials Chemistry

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 96 hits
ReferencesLink to record
Permanent link

Direct link