liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Real time observation of diffusion and bioaffinity binding processes in single polyelectrolyte-coated microcapsules: A fluorescence-based approach
National University of Singapore.ORCID iD: 0000-0003-3274-6029
National University of Singapore.
National University of Singapore.
2007 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 60, no 1, 125-130 p.Article in journal (Refereed) Published
Abstract [en]

We report on using fluorescence microscopy to study, visualize and determine the diffusion phenomena into and bioaffinity binding within single microcapsules in real time by using biotin–fluorescein as diffusive species and encapsulated avidin as binding partner. Microcapsules were constructed by entrapment of avidin within an agarose matrix and encapsulated with polyelectrolyte layers by Layer-by-Layer (LbL) polyelectrolyte self assembly. A “ring” of high fluorescence intensity advancing with time towards the capsule centre was observed during incubation of capsules with fluorescent-labeled biotin. Fluorescence intensity was build up in capsule areas where binding to avidin occurred and was visualized in real time. A model for the diffusion process in microcapsules was developed and experimental data was plotted and fitted well with trends predicted by the model. The value of the diffusion coefficient for biotin–fluorescein was determined to be 3.5 × 10−8 cm2/s, which is comparable to literature values of similar sized molecules.

Place, publisher, year, edition, pages
Elsevier, 2007. Vol. 60, no 1, 125-130 p.
Keyword [en]
Microcapsule diffusion; Biotin avidin; Layer-by-layer; Polyelectrolyte
National Category
Biomaterials Science
URN: urn:nbn:se:liu:diva-92070DOI: 10.1016/j.colsurfb.2007.05.019OAI: diva2:620067
Available from: 2013-05-07 Created: 2013-05-07 Last updated: 2015-03-12Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Mak, Wing Cheung
In the same journal
Colloids and Surfaces B: Biointerfaces
Biomaterials Science

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 61 hits
ReferencesLink to record
Permanent link

Direct link