Electrochemical bioassay utilizing encapsulated electrochemical active microcrystal biolabels
2005 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 77, no 9, 2835-2841 p.Article in journal (Refereed) Published
A new approach to perform electrochemical immunoassay based on the utilization of encapsulated microcrystal was developed. The microcrystal labels create a “supernova effect” upon exposure to a desired releasing agent. The microcrystal cores dissolve, and large amounts of signal-generating molecules diffuse across the capsule wall into the outer environment. Layer-by-Layer (LbL) technology was employed for the encapsulation of electrochemical signal-generating microcrystals (ferrocene microcrystals). The encapsulated microcrystals were conjugated with antibody molecules through the adsorption process. The biofunctionalized microcrystals were utilized as a probe for immunoassays. The microcrystal-based label system provided a high-signal molecule to antibody (S/P) ratio of 104−105. Microcrystal biolabels with different antibody surface coverage (1.60−5.05 mg m-2) were subjected to a solid-phase immunoassay for the detection of mouse immunoglobulin G (M-IgG) molecules. The microcrystal-based immunoassay for the detection of M-IgG performed with microcrystals having antibody surface coverage of 5.05 mg m-2 showed a sensitivity of 3.93 nA μg-1 L-1 with a detection limit of 2.82 μg L-1.
Place, publisher, year, edition, pages
American Chemical Society (ACS), 2005. Vol. 77, no 9, 2835-2841 p.
IdentifiersURN: urn:nbn:se:liu:diva-92071DOI: 10.1021/ac048505lOAI: oai:DiVA.org:liu-92071DiVA: diva2:620070