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Nanoscale surface engineered living cells with extended substrate spectrum
Hong Kong University of Science and Technology, Hong Kong, China.ORCID iD: 0000-0003-3274-6029
Hong Kong University of Science and Technology, Hong Kong, China.
Hong Kong University of Science and Technology, Hong Kong, China.
Hong Kong University of Science and Technology, Hong Kong, China.
2004 (English)In: IEE Proceedings - Nanobiotechnology, ISSN 1478-1581, Vol. 151, no 2, 67-72 p.Article in journal (Refereed) Published
Abstract [en]

We report on cell surface engineering of living microorganisms by using Layer-by-Layer (LbL) technology to extend the substrate spectrum. The yeast Arxula adeninivorans LS3 (Arxula) was employed as a model organism and biological template. By using LbL technology, Arxula cells were encapsulated by polyelectrolyte and enzyme layers. The biological activity of the Arxula was retained after the encapsulation process. The polymeric capsule surrounding the Arxula provides a stable interface for surface engineering of living cells. LbL of polyelectrolytes followed by an enzyme layer of lactate oxidase were assembled. The outer enzyme layer provides an additional biological function for Arxula to convert the unfavourable substrate lactate into the favourable substrate pyruvate, thus extending the substrate spectrum of the organism. Moreover, capsule stability and enzyme conjugate stability of the surface engineered Arxula were studied.

Place, publisher, year, edition, pages
IEEE , 2004. Vol. 151, no 2, 67-72 p.
National Category
Nano Technology
Identifiers
URN: urn:nbn:se:liu:diva-92074DOI: 10.1049/ip-nbt:20040535PubMedID: 16475845OAI: oai:DiVA.org:liu-92074DiVA: diva2:620075
Available from: 2013-05-07 Created: 2013-05-07 Last updated: 2015-03-12Bibliographically approved

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Mak, Wing Cheung

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CiteExportLink to record
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Citation style
  • apa
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  • Other style
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  • de-DE
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  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
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