Development of a gold nano-particle-based fluorescent molecular beacon fordetection of cystic fibrosis associated mutation
2010 (English)In: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 26, no 2, 307-313 p.Article in journal (Refereed) Published
Cystic fibrosis is one of the most common genetically inherited diseases in Northern Europe, consistingof an inherited defect of chloride transport in the epithelium. Of the several mutations related to CF, theF508 mutation occurs in ca. 70% of the cases. In this work the use of a gold nano-particle supportedfluorescence molecular beacon was investigated as an optical sensing platform for the detection of theF508 cystic fibrosis associated mutation. Different parameters such as molecular beacon design, Aunano-particle size, molecular beacon–nano-particle conjugation protocol, molecular beacon loading aswell as experimental conditions were evaluated. A 31-base long molecular beacon, containing a 15-baserecognition sequence specific for the mutant target, was linked via a thiol modified poly thymine linker(10 bases long) to a 13 nm gold nano-particle and was exposed to mutant and wild type targets, and aclear differentiation was achieved at target concentrations as low as 1 nM.
Place, publisher, year, edition, pages
Elsevier, 2010. Vol. 26, no 2, 307-313 p.
Molecular beacon, Cystic fibrosis, F508, Au nano-particle, DNA sensor
IdentifiersURN: urn:nbn:se:liu:diva-92096DOI: 10.1016/j.bios.2010.08.043PubMedID: 20829026OAI: oai:DiVA.org:liu-92096DiVA: diva2:620121