Electrochemical melting-curve analysis.
2010 (English)In: Electrochemistry communications, ISSN 1388-2481, E-ISSN 1873-1902, Vol. 12, 1030-1033 p.Article in journal (Refereed) Published
Genotyping technologies need to tackle issues of cost-effectiveness, flexibility, and mutiplexability to meetthe ever-increasing demands for clinical diagnostics, addressing the future medical paradigm. Here wereport on a facile method for the rapid detection of mutations using electrochemical melting-curve analysis.The concept is based on the use of an immobilised probe hybridised to the mutant region of a ferrocenelabelled amplicon. Following hybridisation, the temperature is ramped and the dissociation of the ferrocenelabelled DNA from the electrode surface is monitored using differential pulse voltammetry. Using a modelsystem consisting of short probe and target, the proposed approach was demonstrated to clearlydiscriminate between complementary and mismatch duplexes. The melting temperature of the surfaceconfined DNA duplex was observed to be markedly lower than that obtained in solution, with meltingtemperatures of 38 and 59 °C, observed, respectively. The approach can be extended to array based meltingcurveanalysis, allowing the simultaneous detection of multiple mutations, as well as for genosensor design.
Place, publisher, year, edition, pages
Elsevier, 2010. Vol. 12, 1030-1033 p.
Electrochemical melting-curve analysis, Electrochemical genosensor, Electrochemical mutation detection, Cystic fibrosis
IdentifiersURN: urn:nbn:se:liu:diva-92137OAI: oai:DiVA.org:liu-92137DiVA: diva2:620235