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Effects of prolactin on platelet activation and blood clotting
Linköping University, Department of Medical and Health Sciences, Internal Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Heart and Medicine Center, Department of Endocrinology.
Linköping University, Department of Clinical and Experimental Medicine. Linköping University, Faculty of Health Sciences.
Östergötlands Läns Landsting, Heart and Medicine Center, Department of Endocrinology. Linköping University, Department of Medical and Health Sciences, Division of Cardiovascular Medicine. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Clinical and Experimental Medicine, Clinical Chemistry. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Chemistry.
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2013 (English)In: Scandinavian Journal of Clinical and Laboratory Investigation, ISSN 0036-5513, E-ISSN 1502-7686, Vol. 73, no 3, 221-228 p.Article in journal (Refereed) Published
Abstract [en]

Increased levels of prolactin often coincide with an increased risk for thromboembolic events, but it is unclear whether a direct causal relation exists. Our aim was to examine the effect of prolactin on platelet function. In addition to using recombinant prolactin for experiments in vitro, we analyzed platelet function by flow cytometry in a group of 13 females with hyperprolactinaemia and 18 healthy female controls. Platelet activation was measured by P-selectin expression and by the amount of platelet-bound fibrinogen after stimulation with adenosine diphosphate (ADP), collagen-related peptide and the protease activated receptor (thrombin receptor) (PAR)-activating peptides PAR4-AP and PAR1-AP. Free oscillation rheometry was used to measure clotting time in whole blood. No significant effect on platelet activation or clotting time could be seen in in vitro experiments by adding recombinant prolactin. However, significantly lower P-selectin expression was found in the hyperprolactinemic group when platelets were activated by ADP (5 and 10 mu M) or PAR4-AP. The expression of fibrinogen did not differ between the two groups for any of the activators used. For all samples, inverse significant correlations between P-selectin expression and prolactin concentration were found for both 5 mu M ADP (r = 0.61, p andlt; 0.01), 10 mu M ADP (r = -0.62, p andlt; 0.001) and PAR4-AP (r = -0.69, p andlt; 0.001). Thrombin cleavage of recombinant prolactin resulting in a 16 kDa C-terminal fragment did not alter the P-selectin expression upon activation. We found an indirect inhibitory effect of prolactin on platelets in hyperprolactinemic patients, suggesting that prolactin might have a protective role in thromboembolic disease.

Place, publisher, year, edition, pages
Informa Healthcare , 2013. Vol. 73, no 3, 221-228 p.
Keyword [en]
Prolactin, platelets, P-selectin, fibrinogen, prolactinoma
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-92702DOI: 10.3109/00365513.2013.765963ISI: 000317486400005OAI: oai:DiVA.org:liu-92702DiVA: diva2:621664
Note

Funding Agencies|FORSS (Medical Research Council of Southeast Sweden)|4065|Faculty of Health and Sciences, Linkoping University, Sweden||

Available from: 2013-05-16 Created: 2013-05-16 Last updated: 2017-12-06

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Wahlberg Topp, JeanetteTillmar, LindaEkman, BertilLindahl, TomasLandberg, Eva

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Wahlberg Topp, JeanetteTillmar, LindaEkman, BertilLindahl, TomasLandberg, Eva
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Internal MedicineFaculty of Health SciencesDepartment of EndocrinologyDepartment of Clinical and Experimental MedicineDivision of Cardiovascular MedicineClinical ChemistryDepartment of Clinical Chemistry
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Scandinavian Journal of Clinical and Laboratory Investigation
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