DEVELOPMENT AND VALIDATION OF RP-HPLC-DAD METHOD FOR DETERMINATION OF NINE DRUGS AND THEIR ELEVEN METABOLITES IN PLASMA AND URINE: PLASMA SAMPLES MEASUREMENTS
2013 (English)In: Journal of Liquid Chromatography & Related Technologies, ISSN 1082-6076, E-ISSN 1520-572X, Vol. 36, no 12, 1597-1615 p.Article in journal (Refereed) Published
A new RP-HPLC-DAD method for determination of nine drugs and eleven metabolites in body fluids was developed. The separation of drugs and metabolites (PAR, SOT, MET, ASP, PRO, NIF, CAR, DEX, KET, PAR-S, PAR-G, MET-H, D-MET, SAL, GENT, PRO-S, D-NIF, ODMC, O-DEX, and KET-G) was achieved using Develosil RP-AQUEOUS-AR5 C30 column in 30min. The obtained MQL values (0.02 mu g/mL0.23 mu g/mL) are suitable for urine/plasma measurements. Correlation coefficient (r(2)) was higher than 0.98 for all analytes. The recoveries ranged from 72.1% to 115.2% (RSD andlt;6.4%). Chromatographic data were obtained with accuracy in the range from 0.3 to 5.6%.
Place, publisher, year, edition, pages
Taylor and Francis: STM, Behavioural Science and Public Health Titles / Taylor and Francis , 2013. Vol. 36, no 12, 1597-1615 p.
drugs, HPLC-DAD, metabolites, plasma, solid phase extraction, urine
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-93399DOI: 10.1080/10826076.2012.695309ISI: 000318071500001OAI: oai:DiVA.org:liu-93399DiVA: diva2:624476