Enhanced Fluorescent Assignment of Protein Aggregates by an Oligothiophene-Porphyrin-Based Amyloid Ligand
2013 (English)In: Macromolecular rapid communications, ISSN 1022-1336, E-ISSN 1521-3927, Vol. 34, no 9, 723-730 p.Article in journal (Refereed) Published
Fluorescent probes identifying protein aggregates are of great interest, as deposition of aggregated proteins is associated with many devastating diseases. Here, we report that a fluorescent amyloid ligand composed of two distinct molecular moieties, an amyloidophilic pentameric oligothiophene and a porphyrin, can be utilized for spectral and lifetime imaging assessment of recombinant A 1-42 amyloid fibrils and A deposits in brain tissue sections from a transgenic mouse model with Alzheimers disease pathology. The enhanced spectral range and distinct lifetime diversity of this novel oligothiopheneporphyrin-based ligand allow a more precise assessment of heterogeneous amyloid morphology compared with the corresponding oligothiophene dye.
Place, publisher, year, edition, pages
Wiley-VCH Verlag , 2013. Vol. 34, no 9, 723-730 p.
oligothiophene, porphyrin, protein deposits, imaging, fluorescence
Engineering and Technology
IdentifiersURN: urn:nbn:se:liu:diva-93385DOI: 10.1002/marc.201200817ISI: 000318354500004OAI: oai:DiVA.org:liu-93385DiVA: diva2:624499
Funding Agencies|Swedish Research Council||Knut and Alice Wallenberg Foundation||Swedish Foundation for Strategic Research||European Union FP7 HEALTH (Project LUPAS)||LiU Neuroscience Center||ERC Starting Independent Researcher grant (Project: MUMID)||2013-05-312013-05-312015-05-28