liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Sperm chromatin structure and sperm morphology: Their association with fertility in AI-dairy Ayrshire sires
University of Pannonia, Hungary .
Swedish University of Agriculture Science SLU, Sweden .
Faba Coop, Finland .
Viking Genet Finland, Finland .
Show others and affiliations
2013 (English)In: Theriogenology, ISSN 0093-691X, E-ISSN 1879-3231, Vol. 79, no 8, 1153-1161 p.Article in journal (Refereed) Published
Abstract [en]

Prognostic relations between sperm variables and sire fertility are yet elusive. A retrospective analysis of sperm morphology and chromatin stability (studied using sperm chromatin structure assay [SCSA]) and their relation to fertility after AI (as proportions of 60 days of nonreturn to estrous [NRR], corrected NRR, or calving rate) was studied with preselected frozen semen doses from a group (N = 43) of AI-sires of the Finnish Ayrshire breed composed of 50% subfertile bulls (andlt;55% NRR) and 50% fertile sires (andgt;55% NRR). Fertility, indicated by all three parameters, correlated significantly only with the percentage of morphologically normal spermatozoa, a variable which negatively correlated with the percentage of DNA fragmentation at the time of SCSA, thus confirming the value of always having high numbers of morphologically normal spermatozoa in AI-doses. Proportions of major sperm defects also related to fertility but only when considering corrected NRR, not with calving rate, indicating that proportions of normal spermatozoa, a value surpassing differences between sperm laboratory screening methods, might be valuable and could be easily made routine by the industry. Though SCSA as a method is being contested for DNA- and chromatin analyses in the light of epigenetic changes, a particular parameter, the High Green fluorescence, showed the highest values for sperm doses collected from bulls having meiotic problems and containing a high proportion of diploid spermatozoa (approximately 20%) and also in bulls having a reciprocal chromosomal translocation, thus suggesting such a parameter might be useable to discriminate which bulls ought to be studied in more detail, including cytogenetic analyses.

Place, publisher, year, edition, pages
Elsevier , 2013. Vol. 79, no 8, 1153-1161 p.
Keyword [en]
Bull, Sperm morphology, Sperm chromatin integrity, Fertility
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-93968DOI: 10.1016/j.theriogenology.2013.02.011ISI: 000318750400003OAI: diva2:628272

Funding Agencies|Royal Swedish Academy of Agriculture and Forestry (KSLA)||Knut and Alice Wallenbergs foundation (forskarstipendiefond)||VR, Stockholm||

Available from: 2013-06-13 Created: 2013-06-13 Last updated: 2015-03-12

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Rodriguez-Martinez, Heriberto
By organisation
Division of Microbiology and Molecular MedicineFaculty of Health Sciences
In the same journal
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 86 hits
ReferencesLink to record
Permanent link

Direct link