The origin of naked barley (Hordeum vulgare L. ssp. vulgare) studied bythe nud gene
Independent thesis Basic level (degree of Bachelor), 10,5 credits / 16 HE creditsStudent thesis
The exact origin of the peculiar naked barley is somewhat illusive. There is a debate whether it has a single, monophyletic origin or a multiple, paraphyletic origin. It is from previous Asian studies on naked barley known that a mutation or a deletion of the nud gene expresses the naked seed phenotype. Not much investigation has been done outside of Asia, least of all in the Nordic countries, on what gives naked barley its character. Therefore this study was set up to examine if the Nordic variant of naked barley shares the same nud allele as the Asian and thus has a close connection with it, or if they have independent mutations. I could confirm that the known alleles of the nud gene do determine the seed character of barley. Most of the results of the PCR genotyping confirmed the phenotype of the tested accessions, both naked and hulled barleys. However, one visually phenotyped naked barley cultivar (NGB4580) still amplified with the known primers that would match the Asian hulled allele, meaning that the Nordic accession NGB4580 of naked barley did not carry the known nud deletion. This suggests that naked barley has arisen independently in Asia and in the Nordic countries.
Place, publisher, year, edition, pages
2013. , 14 p.
Accessions, barley landraces, domestication, ethylene response factor, monophyletic origin, nud gene.
IdentifiersURN: urn:nbn:se:liu:diva-94288ISRN: LITH-IFM-G-EX--13/2783–SEOAI: oai:DiVA.org:liu-94288DiVA: diva2:631385
Subject / course
Microbiology and Molecular Genetics
Hagenblad, Jenny, Associate Professor