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Test av litiumheparinplasma för analys av folat med Siemens Advia Centaur® XP samt effekt av inomhusbelysning över tid
Linköping University, Faculty of Health Sciences.
2013 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesis
Abstract [en]

Background: Folate is a water - soluble vitamin necessary for normal DNA synthesis among  other mechanisms. Folate deficiency can cause megaloblastic anemia resulting from abnormal erythrocyte d evelopment. At the Department of Clinical Chemistry, Linköping, analysis of folate is performed with Siemens ADVIA Centaur® XP which uses an immunochemical method with chemiluminimetric detection. Currently, the assay is conducted on serum, is poured off and frozen after 48 h and treated with light protection because folate is considered to be light sensitive.

Methods: This study tested lithium heparin plasma for analysis of folate with Siemens ADVIA Centaur® XP with the aim to be able to perform a package of analyzes in anemia investigation from the same collection tube. Furthermore, the stability of folate was studied for up to 168 h and examined regarding light sensitivity.

Results: Statistically significant higher values were obtained in the analysis of folate in lithium heparin plasma than folate in serum (p < 0,001). Serum folate was found to be stable up to 168 h and plasma folate was stable up to120 h. No statistically significant difference was found either between the serum or plasma folate regarding light exposure.

Conclusion: Folate cannot be analyzed on lithium heparin plasma with the same reference range for serum folate, and further studies and a new reference range for plasma folate are needed to decide whether folate allows to be analyzed in plasma. Collection tubes for determination of folate in serum do not need to be light protected or poured off and frozen after 48 h which simplifies sample handling, reduces errors by relabeling and results in increased prospective automatized handling.

Abstract [sv]

Bakgrund: Folat är ett vattenlösligt vitamin som behövs bland annat för normal DNA-syntes. Folatbrist kan orsaka bland annat megaloblastanemi till följd av onormal erytrocytmognad. Analys av folat utförs vid Klinisk kemi med analysinstrumentet Siemens ADVIA Centaur® XP med hjälp av en immunokemisk metod med kemiluminimetrisk detektion. Idag utförs analysen på serum, fryses vid analys efter 48 h och behandlas ljusskyddat eftersom folat anses vara ljuskänsligt.

Metod: I denna studie testades litiumheparinplasma för analys av folat med Siemens ADVIA Centaur® XP för att kunna utföra ett paket av analyser inom anemiutredning på samma provtagningsrör. Dessutom studerades stabiliteten upp till 168 h samt effekten av inomhusbelysning.

Resultat: Vid analys av folat i litiumheparinplasma erhölls statistiskt signifikant högre resultat än vid analys av folat i serum (p < 0,001). Serumfolat visade sig vara stabilt upp till och med 168 h och plasmafolat var stabilt fram till 120 h. Ingen statistisk signifikant skillnad fanns vare sig mellan serumfolat eller plasmafolat avseende ljusexponering.

Konklusion: Folat kan inte analyseras på litiumheparinplasma jämställt med serum med samma referensintervallutan fler studier behövs ochnytt referensintervall för plasmafolat bör tas fram. Prov för bestämning av folat i serum behöver inte ljusskyddas eller hällas av och frysas efter 48 h vilket underlättar provhanteringen, minskar felkällor med ommärkning och medför att prov därmed i framtiden kan hanteras mera automatiserat.

Place, publisher, year, edition, pages
2013. , 20 p.
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:liu:diva-95880ISRN: LIU-HU/BML-G—13/011—SEOAI: oai:DiVA.org:liu-95880DiVA: diva2:639050
Subject / course
Biomedical Laboratory Science
Available from: 2013-09-12 Created: 2013-08-05 Last updated: 2014-01-10Bibliographically approved

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Citation style
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