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Potentiometric urea biosensor utilizing nanobiocomposite of chitosan-iron oxide magnetic nanoparticles
Linköping University, Department of Science and Technology. Linköping University, The Institute of Technology. Bahauddin Zakriya University, Multan, Pakistan.
King Saud University, Riyadh, Saudi Arabia and Bahauddin Zakriya University, Multan, Pakistan.
King Saud University, Riyadh, Saudi Arabia.
King Saud University, Riyadh, Saudi Arabia.
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2013 (English)In: Journal of Physics, Conference Series, ISSN 1742-6588, E-ISSN 1742-6596, Vol. 414Article in journal (Refereed) Published
Abstract [en]

The iron oxide (Fe3O4) magnetic nanoparticles have been fabricated through a simple, cheap and reproducible approach. Scanning electron microscope, x-rays powder diffraction of the fabricated nanoparticles. Furthermore, the fabrication of potentiometric urea biosensor is carried out through drop casting the initially prepared isopropanol and chitosan solution, containing Fe3O4 nanoparticles, on the glass fiber filter with a diameter of 2 cm and a copper wire (of thickness −500 μm) has been utilized to extract the voltage signal from the functionalized nanoparticles. The functionalization of surface of the Fe3O4 nanoparticles is obtained by the electrostatically immobilization of urease onto the nanobiocomposite of the chitosan- Fe3O4 in order to enhance the sensitivity, specificity, stability and reusability of urea biosensor. Electrochemical detection procedure has been adopted to measure the potentiometric response over the wide logarithmic concentration range of the 0.1 mM to 80 mM. The Fe3O4 nanoparticles based urea biosensor depicts good sensitivity with ~42 mV per decade at room temperature. Durability of the biosensor could be considerably enhanced by applying a thin layer of the nafion. In addition, the reasonably stable output response of the biosensor has been found to be around 12 sec.

Place, publisher, year, edition, pages
Institute of Physics (IOP), 2013. Vol. 414
Keyword [en]
iron oxide (Fe3O4) magnetic nanoparticles; emission characteristics; potentiometric response; urea biosensor; chitosan solution
National Category
Natural Sciences
URN: urn:nbn:se:liu:diva-96519DOI: 10.1088/1742-6596/414/1/012024ISI: 000315404300024OAI: diva2:642359
21st International Laser Physics Workshop (LPHYS 2012), 23-27 July 2012, Calgary, Canada
Available from: 2013-08-21 Created: 2013-08-20 Last updated: 2014-01-15

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