liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
The Structural Basis for Optimal Performance of Oligothiophene-Based Fluorescent Amyloid Ligands: Conformational Flexibility is Essential for Spectral Assignment of a Diversity of Protein Aggregates
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
Linköping University, Department of Physics, Chemistry and Biology, Chemistry. Linköping University, The Institute of Technology.
Washington University, MO USA .
Show others and affiliations
2013 (English)In: Chemistry - A European Journal, ISSN 0947-6539, E-ISSN 1521-3765, Vol. 19, no 31, 10179-10192 p.Article in journal (Refereed) Published
Abstract [en]

Protein misfolding diseases are characterized by deposition of protein aggregates, and optical ligands for molecular characterization of these disease-associated structures are important for understanding their potential role in the pathogenesis of the disease. Luminescent conjugated oligothiophenes (LCOs) have proven useful for optical identification of a broader subset of disease-associated protein aggregates than conventional ligands, such as thioflavin T and Congo red. Herein, the molecular requirements for achieving LCOs able to detect nonthioflavinophilic Aβ aggregates or non-congophilic prion aggregates, as well as spectrally discriminate Aβ and tau aggregates, were investigated. An anionic pentameric LCO was subjected to chemical engineering by: 1) replacing thiophene units with selenophene or phenylene moieties, or 2) alternating the anionic substituents along the thiophene backbone. In addition, two asymmetric tetrameric ligands were generated. Overall, the results from this study identified conformational freedom and extended conjugation of the conjugated backbone as crucial determinants for obtaining superior thiophene-based optical ligands for sensitive detection and spectral assignment of disease-associated protein aggregates.

Place, publisher, year, edition, pages
Wiley-VCH Verlag , 2013. Vol. 19, no 31, 10179-10192 p.
Keyword [en]
Alzheimers disease, fluorescent probes, luminescent conjugated oligothiophenes, microscopy, protein folding
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-96708DOI: 10.1002/chem.201301463ISI: 000321983700020OAI: oai:DiVA.org:liu-96708DiVA: diva2:642973
Note

Funding Agencies|Swedish Foundation for Strategic Research||European Research Council||NIH|P50AG056815PO1-AG03991|

Available from: 2013-08-23 Created: 2013-08-23 Last updated: 2017-12-06

Open Access in DiVA

fulltext(1527 kB)275 downloads
File information
File name FULLTEXT01.pdfFile size 1527 kBChecksum SHA-512
696a568096cbf5f7b5f5ea0b4d742bf752280d25c642b0c1f380e4509d70726ba827e041e467012624505b52a4f726de3c0b6543f11bb4d338083af530b35617
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Klingstedt, TheréseShirani, HamidÅslund, AndreasNilsson, Peter

Search in DiVA

By author/editor
Klingstedt, TheréseShirani, HamidÅslund, AndreasNilsson, Peter
By organisation
ChemistryThe Institute of Technology
In the same journal
Chemistry - A European Journal
Engineering and Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 275 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 131 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf