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Gradients in surface nanotopography used to study platelet adhesion and activation
University of Gothenburg, Sweden .
Chalmers, Sweden .
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Chemistry.
Linköping University, Department of Clinical and Experimental Medicine, Division of Microbiology and Molecular Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Chemistry.
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2013 (English)In: Colloids and Surfaces B: Biointerfaces, ISSN 0927-7765, E-ISSN 1873-4367, Vol. 110, 261-269 p.Article in journal (Refereed) Published
Abstract [en]

Gradients in surface nanotopography were prepared by adsorbing gold nanoparticles on smooth gold substrates using diffusion technique. Following a sintering procedure the particle binding chemistry was removed, and integration of the particles into the underlying gold substrate was achieved, leaving a nanostructured surface with uniform surface chemistry. After pre-adsorption of human fibrinogen, the effect of surface nanotopography on platelets was studied. The use of a gradient in nanotopography allowed for platelet adhesion and activation to be studied as a function of nanoparticle coverage on one single substrate. A peak in platelet adhesion was found at 23% nanoparticle surface coverage. The highest number of activated platelets was found on the smooth control part of the surface, and did not coincide with the number of adhered platelets. Activation correlated inversely with particle coverage, hence the lowest fraction of activated platelets was found at high particle coverage. Hydrophobization of the gradient surface lowered the total number of adhering cells, but not the ratio of activated cells. Little or no effect was seen on gradients with 36 nm particles, suggesting the existence of a lower limit for sensing of surface nano-roughness in platelets. These results demonstrate that parameters such as ratio between size and inter-particle distance can be more relevant for cell response than wettability on nanostructured surfaces. The minor effect of hydrophobicity, the generally reduced activation on nanostructured surfaces and the presence of a cut-off in activation of human platelets as a function of nanoparticle size could have implications for the design of future blood-contacting biomaterials.

Place, publisher, year, edition, pages
Elsevier , 2013. Vol. 110, 261-269 p.
Keyword [en]
Nanoparticles, Nanotopography, Gradient, Fibrinogen, Platelet activation
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-96698DOI: 10.1016/j.colsurfb.2013.04.010ISI: 000321940200035OAI: oai:DiVA.org:liu-96698DiVA: diva2:642982
Note

Funding Agencies|BIOMATCELL VINN excellence center for biomaterial and cell therapy at Gothenburg University||Swedish Research Council|VR 2009-5398|

Available from: 2013-08-23 Created: 2013-08-23 Last updated: 2017-12-06

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Faxälv, LarsLindahl, Tomas

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Division of Microbiology and Molecular MedicineFaculty of Health SciencesDepartment of Clinical Chemistry
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