liu.seSearch for publications in DiVA
Change search
ReferencesLink to record
Permanent link

Direct link
Differences in the expression of hepatocyte growth factor in acute and chronic bowel inflammation - Implications for diagnosis?
PEAS Institut, Linköping, Sweden.
PEAS Institut, Linköping, Sweden.
Linköping University, Department of Clinical and Experimental Medicine, Division of Inflammation Medicine. Linköping University, Faculty of Health Sciences. Östergötlands Läns Landsting, Center for Diagnostics, Department of Clinical Pathology and Clinical Genetics.
Örebro University, Sweden.
Show others and affiliations
2013 (English)In: Advances in Bioscience and Biotechnology, ISSN 2156-8456, E-ISSN 2156-8502, Vol. 4, no 8A2, 33-42 p.Article in journal (Refereed) Published
Abstract [en]

Background: Hepatocyte growth factor (HGF) acts as an acute phase protein with regenerative properties. HGF is produced systemically and locally during inflammation but exhibits decreased binding affinity to heparan sulphate proteoglycan (HSPG)/glycosaminoglycan during chronic inflammation. We previously observed a high faecal concentration and binding affinity of HGF to HSPG during acute gastroenteritis. High faecal concentrations of calprotectin and HGF have been reported in chronic inflammatory bowel disease (IBD).

Methods: Stool samples from patients with ulcerative colitis in remission (n = 11) or exacerbation (n = 5), microscopic colitis (n = 11), colon cancer (n = 6), or acute gastroenteritis caused by Clostridium difficile (n = 20), as well as healthy controls (n = 7), were analysed for the presence of HGF by ELISA, surface plasmon resonance, SDS-PAGE, and Western blot. Then in two patients with ulcerative colitis exacerbation and C. difficile infection, the expression of HGF and calprotectin was studied in colonic biopsies.

Results: The faecal concentration of HGF was significantly higher in patients with ulcerative colitis compared to the other groups. The binding affinity to dextran was lower in all groups compared to acute inflammation. HGF receptor binding was similar across groups. In a patient with concomitant C. difficile infection and distal ulcerative colitis, HGF was highly expressed in the part of the bowel unaffected by ulcerative colitis, but no expression was found at the site of chronic inflammation. In the patient with total colitis the biopsies showed low expression of HGF. The areas with chronic inflammation exhibited infiltrating calprotectin-stained neutrophils.

Conclusion: HGF is produced locally during inflammation of the bowel. The HGF produced during acute inflammation or exacerbations of chronic inflammation by the unaffected area shows binding affinity to glucosaminoglycans. Measuring HGF binding in faeces and biopsies may be a tool for differentiating between acute and chronic bowel inflammation, which should be assessed thoroughly in future studies.

Place, publisher, year, edition, pages
Scientific Research Publishing, 2013. Vol. 4, no 8A2, 33-42 p.
Keyword [en]
Diarrhoea; Ulcerative Colitis; Dextran Sulphate; HGF; HSPG; Calprotectin
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-96964DOI: 10.4236/abb.2013.48A2006OAI: diva2:644791
Available from: 2013-09-02 Created: 2013-09-02 Last updated: 2014-08-20Bibliographically approved

Open Access in DiVA

fulltext(1679 kB)132 downloads
File information
File name FULLTEXT01.pdfFile size 1679 kBChecksum SHA-512
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Search in DiVA

By author/editor
Lönn, JohannaOlsson, HansAlmer, Sven
By organisation
Division of Inflammation MedicineFaculty of Health SciencesDepartment of Clinical Pathology and Clinical GeneticsGastroenterology and HepatologyDepartment of GastroentorologyInfectious DiseasesDepartment of Infectious Diseases
In the same journal
Advances in Bioscience and Biotechnology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
Total: 132 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 122 hits
ReferencesLink to record
Permanent link

Direct link