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A distributed image-processing system for measurements of intracellular calcium in living cells
Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.ORCID iD: 0000-0002-3756-207X
Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
1991 (English)In: Computer Methods and Programs in Biomedicine, ISSN 0169-2607, E-ISSN 1872-7565, Vol. 36, no 4, p. 199-221Article in journal (Refereed) Published
Abstract [en]

During the last decade, image-processing techniques have been introduced as a valuable tool in biologically oriented research. In combination with novel fluorescent probes, these techniques permit assessment of subcellular distributions of several intracellularly important cations, such as free calcium ions and protons. Typically, systems used for image processing are located centrally around the experimental setup. This configuration has drawbacks, mainly because the laborious extraction and processing of data that generally follow an experimental session limits the access to the system for other investigators. We describe here the principles of a distributed image processing system, based on IBM-compatible personal computers (PCs), that without extra hardware can cope with all the necessary image processing involved in imaging of intracellular cations. The potential of the PC as an image processor, however, reaches beyond this specific application and many image processing tasks can be carried out successfully on a standard PC. Thus, the centrally located dedicated image processor is used only for image acquisition in the experimental situation. This in turn optimizes the utilization of expensive resources and increases efficiency. The mouse-operated software is described in detail, so that interested investigators can extract useful parts for integration into their own applications and experimental environment.

Place, publisher, year, edition, pages
Elsevier, 1991. Vol. 36, no 4, p. 199-221
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-98030DOI: 10.1016/0169-2607(91)90090-GISI: A1991GX32100006PubMedID: 1773608OAI: oai:DiVA.org:liu-98030DiVA, id: diva2:651324
Available from: 2013-09-25 Created: 2013-09-25 Last updated: 2017-12-06Bibliographically approved

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Gustafsson, MikaelMagnusson, Karl-Eric

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CiteExportLink to record
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Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf