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Roles of Ca2+ and F-actin in intracellular aggregation of Chlamydia trachomatis in eucaryotic cells
Linköping University, Department of Molecular and Clinical Medicine, Clinical Microbiology. Linköping University, Faculty of Health Sciences.
Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.ORCID iD: 0000-0002-3756-207X
Department of clinical microbiology, Örebro medical center hospital, Örebro, Sweden.
Linköping University, Department of Molecular and Clinical Medicine, Medical Microbiology. Linköping University, Faculty of Health Sciences.
1993 (English)In: Infection and Immunity, ISSN 0019-9567, E-ISSN 1098-5522, Vol. 61, no 4, 1406-1414 p.Article in journal (Refereed) Published
Abstract [en]

The effect of intracellular free Ca2+ ([Ca2+]i) on the intracellular aggregation of Chlamydia trachomatis serovars L2 and E in McCoy and HeLa cells is investigated. Loading the cells with the Ca2+ chelator MAPT/AM (1,2-bis-5-methyl-amino-phenoxylethane-N,N-n'-tetra-acetoxymethyl acetate), thereby decreasing the [Ca2+]i from 67 to 19 nM, decreased the number of cells with a local aggregation of chlamydiae in a dose-dependent manner. Neither the attachment nor the uptake of elementary bodies (EBs) was, however, affected after depletion of Ca2+ from the cells. There was no significant difference in the level of measured [Ca2+]i between infected and uninfected cells. Reducing the [Ca2+]i also significantly inhibited chlamydial inclusion formation. Differences in the organization of the actin filament network were observed in response to [Ca2+]i depletion. In Ca(2+)-depleted cells, where few EB aggregates were formed, few local accumulations of F-actin were observed in the cytosol. These results suggest that the aggregation of EBs in eucaryotic cells requires a normal homeostasis of intracellular Ca2+. By affecting F-actin reorganization and putatively certain Ca(2+)-binding proteins, [Ca2+]i plays a vital role in the infectious process of chlamydiae.

Place, publisher, year, edition, pages
American Society for Microbiology , 1993. Vol. 61, no 4, 1406-1414 p.
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Medical and Health Sciences
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URN: urn:nbn:se:liu:diva-98031PubMedID: 8454343OAI: oai:DiVA.org:liu-98031DiVA: diva2:651328
Available from: 2013-09-25 Created: 2013-09-25 Last updated: 2017-12-06Bibliographically approved

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Majeed, MeythamGustafsson, MikaelKihlström, ErikStendahl, Olle

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