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A novel branching pattern in the lipopolysaccharide expressed by non-typeable Haemophilus influenzae strain 1232
Karolinska Institute, Sweden .
National Research Council Canada, Canada .
MRC Harwell, England .
University of Oxford Nuffield, England .
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2013 (English)In: Carbohydrate Research, ISSN 0008-6215, E-ISSN 1873-426X, Vol. 378, 114-122 p.Article in journal (Refereed) Published
Abstract [en]

We report the novel branching pattern in lipopolysaccharide (LPS) expressed by non-typeable Haemophilus influenzae (NTHi) strain 1232. The strain expressed the beta-D-Glcp-(1 -andgt; 4)-[alpha-D-Galp-(1 -andgt; 4)-beta-D-Galp-(1 -andgt; 7)]-D-alpha-D-Hepp-(1 -andgt; 6)-beta-D-Glcp chain linked to the proximal heptose (HepI) of the conserved triheptosyl inner-core moiety of NTHi LPS: L-alpha-D-HepIIIp-(1 -andgt; 2)-[PEtn -andgt; 6]-L-alpha-D-HepIIp-(1 -andgt; 3)-L-alpha-D-HepIp-(1 -andgt; 5)-[PPEtn -andgt; 4]-alpha-Kdop-(2 -andgt; 6)-lipid A. The structure has been elucidated using NMR spectroscopy, electrospray ionization mass spectrometry (ESI-MS) and capillary electrophoresis coupled to electrospray ionization tandem mass spectrometry (CE-ESI-MSn) on O-deacylated LPS and core oligosaccharide (OS) materials, as well as HPLC-ESI-MSo on permethylated, dephosphorylated OS. It was also found that a tetrasaccharide unit bearing sialic acid [alpha-Neu5Ac-(2 -andgt; 3)-beta-D-Galp-(1 -andgt; 4)-beta-D-GlcNAcp-(1 -andgt; 3)-beta-D-Galp-(1 -andgt;] could substitute O-4 of the beta-D-Glcp linked to HepI. In addition, the distal heptose (HepIII) was substituted by PCho -andgt; 6-beta-D-Galp-(1 -andgt; at the O-2 position.

Place, publisher, year, edition, pages
Elsevier , 2013. Vol. 378, 114-122 p.
Keyword [en]
Haemophilus influenzae, Lipopolysaccharide, Sialyllacto-N-neotetraose, D-glycero-D-manno-Heptose losA1
National Category
Engineering and Technology
Identifiers
URN: urn:nbn:se:liu:diva-98668DOI: 10.1016/j.carres.2013.03.011ISI: 000324299900016OAI: oai:DiVA.org:liu-98668DiVA: diva2:655265
Available from: 2013-10-10 Created: 2013-10-10 Last updated: 2017-12-06

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Schweda, Elke

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