Overexpression of thioredoxin reductase 1 inhibits migration of HEK-293 cells
2007 (English)In: Biology of the Cell, ISSN 0248-4900, E-ISSN 1768-322X, Vol. 99, no 12, 677-687 p.Article in journal (Refereed) Published
TrxR (thioredoxin reductase), in addition to protecting against oxidative stress, plays a role in the redox regulation of intracellular signalling pathways controlling, among others, cell proliferation and apoptosis. The aim of the present study was to determine whether TrxR1 is involved in the regulation of cell migration.
Stably transfected HEK-293 (human embryonic kidney) cells which overexpress cytosolic TrxR1 (HEK-TrxR15 and HEK-TrxR11 cells) were used in the present study. We found that the stimulation of cell motility induced by PKC (protein kinase C) activators, PMA and DPhT (diphenyltin), was inhibited significantly in the HEK-TrxR15 and HEK-TrxR11 cells compared with control cells. The overexpression of TrxR1 also inhibited characteristic morphological changes and reorganization of the F-actin cytoskeleton induced by PMA and DPhT. In addition, the selective activation of PKCdelta by DPhT was inhibited in cells that overexpressed cytosolic TrxR1. Furthermore, rottlerin, a selective inhibitor of PKCdelta, and PKCdelta siRNA (small interfering RNA), suppressed the morphological changes induced by DPhT in the control cells.
The overexpression of TrxR1 inhibits migration of HEK-293 cells stimulated with PMA and DPhT. Moreover, our observations suggest that this effect is mediated by the inhibition of PKCdelta activation.
Place, publisher, year, edition, pages
John Wiley & Sons, 2007. Vol. 99, no 12, 677-687 p.
cell migration, diphenyltin, protein kinase C (PKC), redox regulation, thioredoxin reductase (TrxR)
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-98713DOI: 10.1042/BC20070024ISI: 000251443100002PubMedID: 17581112OAI: oai:DiVA.org:liu-98713DiVA: diva2:655533