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S-glutathionylation of IRF3 regulates IRF3-CBP interaction and activation of the IFN beta pathway
Center of Basic Research I—Biochemistry Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
Center of Basic Research I—Biochemistry Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
Center of Basic Research II—Molecular Biology Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
Center of Basic Research I—Biochemistry Division, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
2008 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 27, no 6, 865-875 p.Article in journal (Refereed) Published
Abstract [en]

Interferon regulatory factor 3 (IRF3) is an essential transcriptional regulator of the interferon genes. IRF3 is constitutively present in a latent conformation in the cell cytoplasm. In cells infected by Sendai virus, IRF3 becomes phosphorylated, homodimerizes, translocates to the nucleus, binds to target genes and activates transcription by interacting with CBP/p300 co-activators. In this study, we report that in non-infected cells IRF3 is post-translationally modified by S-glutathionylation. Upon viral-infection, it undergoes a deglutathionylation step that is controlled by the cytoplasmic enzyme glutaredoxin-1 (GRX-1). In virus-infected GRX-1 knockdown cells, phosphorylation, homodimerization and nuclear translocation of IRF3 were not affected, but the transcriptional activity of IRF3 and the expression of interferon-beta (IFNbeta), were severely reduced. We show that deglutathionylation of IRF3 is necessary for efficient interaction of IRF3 with CBP, an event essential for transcriptional activation of the interferon genes. Taken together, these findings reveal a crucial role for S-glutathionylation and GRX-1 in controlling the activation of IRF3 and IFNbeta gene expression.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2008. Vol. 27, no 6, 865-875 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-98717DOI: 10.1038/emboj.2008.28ISI: 000254179200005PubMedID: 18309294OAI: oai:DiVA.org:liu-98717DiVA: diva2:655540
Available from: 2013-10-11 Created: 2013-10-11 Last updated: 2017-12-06Bibliographically approved

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Spyrou, Giannis

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