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Endoplasmic reticulum stress and mineralization inhibition mechanism by the resinous monomer HEMA
Departments of Endodontics and Basic Sciences, Dental School, University of Athens, Athens, Greece / Biochemistry Division, Foundation for Biomedical Research, Academy of Athens, Athens, Greece.
INSERM UMR 911, CR02, Aix-Marseille Université, Marseille, France.
Aix-Marseille Université, CNRS, ISM UMR 7287, Marseille cedex 09, France.
Departments of Endodontics and Basic Sciences, Dental School, University of Athens, Athens, Greece.
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2013 (English)In: International Endodontic Journal, ISSN 0143-2885, E-ISSN 1365-2591, Vol. 46, no 2, 160-168 p.Article in journal (Refereed) Published
Abstract [en]

AIM: To investigate the expression of two endoplasmic reticulum (ER)-resident key chaperone proteins, ERdj5 and BiP, under the influence of resinous monomers and its relationship with the inhibition of mineralization caused by the monomer 2-hydroxyethyl methacrylate (HEMA).

METHODOLOGY: The ERdj5 and BiP expression was studied in vitro, in primary human pulp cell cultures after treatment with three different HEMA concentrations at different time periods. Subsequently, the expression of both the odontoblast markers dentine sialoprotein (DSP) and osteonectin (OSN) was studied in human pulp cells under the same conditions.

RESULTS: The ERdj5 and BiP expression was upregulated in the pulp cells. DSP and OSN were largely dispersed in the cytoplasm in control cell cultures but accumulated in a perinuclear area after exposure to HEMA. Their expression levels were not affected.

CONCLUSIONS: The increased expression of ERdj5 and BiP may reflect activation of ER stress. DSP and OSN accumulation into the cells may lead to their secretion arrest and inhibition of dentine matrix formation. These events may elucidate the mechanism by which HEMA inhibits the mineralization process.

Place, publisher, year, edition, pages
Wiley-Blackwell, 2013. Vol. 46, no 2, 160-168 p.
Keyword [en]
BiP, dentine sialoprotein, endoplasmic, reticulum stress, ERdj5, HEMA, mineralization, osteonectin, resinous monomers.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-98785DOI: 10.1111/j.1365-2591.2012.02103.xPubMedID: 22889382OAI: diva2:655862
Available from: 2013-10-14 Created: 2013-10-14 Last updated: 2013-10-21Bibliographically approved

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