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Cloning, expression and characterization of mouse spermatid specific thioredoxin-1 gene and protein
Center for Biotechnology, Department of Biosciences at NOVUM, Karolinska Institutet, Huddinge, Sweden.
Queen's University, Kingston, Ontario, Canada.
Center for Biotechnology, Department of Biosciences at NOVUM, Karolinska Institutet, Huddinge, Sweden.
Center for Biotechnology, Department of Biosciences at NOVUM, Karolinska Institutet, Huddinge, Sweden.
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2002 (English)In: Molecular human reproduction, ISSN 1360-9947, E-ISSN 1460-2407, Vol. 8, no 8, 710-718 p.Article in journal (Refereed) Published
Abstract [en]

Thioredoxins are proteins that participate in different cellular processes via redox-mediated reactions. For humans, we have recently described two novel members of this family that display a male germ cell specific expression pattern, named spermatid specific thioredoxin (Sptrx-1 and Sptrx-2 respectively). We report here the cloning and characterization of the mouse Sptrx-1 gene and protein, which are similar to those described for the human orthologue. The mouse Sptrx-1 open reading frame encodes for a protein of 462 aa composed of an N-terminal repetitive domain of a 15 residue motif followed by a C-terminal domain typical of thioredoxins. The mouse Sptrx-1 gene sequence is interrupted by only one intron of 525 bp located in the 5'-UTR, and using fluorescence in-situ hybridization we have mapped its chromosomal location to 17E1.2-1.3. Northern blot analysis identified the testis as the only tissue expressing mouse Sptrx-1 mRNA, and by in-situ hybridization we found a strong labelling in the testicular seminiferous tubules, mostly in the round spermatids. Affinity purified antibodies against human Sptrx-1 crossreacted well with the mouse protein confirming its expression in seminiferous tubules at the later stages of spermiogenesis. Recombinant mouse Sptrx-1 displayed protein disulphide reducing activity in an enzymatic assay coupled to NADPH and thioredoxin reductase. The availability of the mouse Sptrx-1 gene sequence is the first step towards the generation of knock-out mice, whose characterization will provide significant information regarding the in-vivo function of Sptrx-1 and its possible implication in several sperm anomalies.

Place, publisher, year, edition, pages
Oxford University Press, 2002. Vol. 8, no 8, 710-718 p.
Keyword [en]
fibrous sheath, sperm, testis, thioredoxin
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-98802DOI: 10.1093/molehr/8.8.710PubMedID: 12149401OAI: oai:DiVA.org:liu-98802DiVA: diva2:655917
Available from: 2013-10-14 Created: 2013-10-14 Last updated: 2017-12-06Bibliographically approved

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Spyrou, Giannis

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