Thioredoxin-2 (TRX-2) is an essential gene regulating mitochondria-dependent apoptosis
2002 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 21, no 7, 1695-703 p.Article in journal (Refereed) Published
Thioredoxin-2 (Trx-2) is a mitochondria-specific member of the thioredoxin superfamily. Mitochondria have a crucial role in the signal transduction for apoptosis. To investigate the biological significance of Trx-2, we cloned chicken TRX-2 cDNA and generated clones of the conditional Trx-2-deficient cells using chicken B-cell line, DT40. Here we show that TRX-2 is an essential gene and that Trx-2-deficient cells undergo apoptosis upon repression of the TRX-2 transgene, showing an accumulation of intracellular reactive oxygen species (ROS). Cytochrome c is released from mitochondria, while caspase-9 and caspase-3, but not caspase-8, are activated upon inhibition of the TRX-2 transgene. In addition, Trx-2 and cytochrome c are co-immunoprecipitated in an in vitro assay. These results suggest that mitochondrial Trx-2 is essential for cell viability, playing a crucial role in the scavenging ROS in mitochondria and regulating the mitochondrial apoptosis signaling pathway.
Place, publisher, year, edition, pages
Wiley-Blackwell, 2002. Vol. 21, no 7, 1695-703 p.
apoptosis, cytochrome, mitochondria, redox, thioredoxin-2
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-98806DOI: 10.1093/emboj/21.7.1695PubMedID: 11927553OAI: oai:DiVA.org:liu-98806DiVA: diva2:655923