liu.seSearch for publications in DiVA
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Possible involvement of thioredoxin reductase as well as thioredoxin in cellular sensitivity to cis-diamminedichloroplatinum (II)
Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Department of Biological Responses, Institute for Virus Research, Kyoto University, Kyoto, Japan.
Show others and affiliations
1999 (English)In: Free Radical Biology & Medicine, ISSN 0891-5849, E-ISSN 1873-4596, Vol. 27, no 5-6, 504-514 p.Article in journal (Refereed) Published
Abstract [en]

The thioredoxin (TRX) system, composed of nicotinamide adenine dinucleotide phosphate (reduced form), TRX, and TRX reductase (TRXR), has multiple biologic functions via thiol-mediated redox control. In this study, we investigated the relationship between intracellular TRXR levels and cellular sensitivity to cis-diamminedichloroplatinum (II) (CDDP). HeLa, a human cervical carcinoma cell line, cultured with CDDP showed a time- and dose-dependent reduction of intracellular TRXR activity, which was well correlated with the decrease in cell viability after exposure to CDDP. In a cell-free system, CDDP was found to directly inactivate the reduced form of purified human TRXR. The CDDP-resistant variants of HeLa cells, established by continuous exposure to CDDP, exhibited an increased expression and activity of TRXR as well as TRX compared with the parental cells. In addition, sodium selenate, an inhibitor of TRXR, was found to increase the susceptibility to CDDP in the CDDP-resistant cells. Moreover, the HeLa cells transfected with an antisense TRXR RNA expression vector to reduce the intracellular enzyme activity displayed an enhanced sensitivity to CDDP. Taken together with previous reports on TRX, these results indicate the possible involvement of TRXR as well as TRX in the cellular sensitivity and resistance to CDDP.

Place, publisher, year, edition, pages
Elsevier, 1999. Vol. 27, no 5-6, 504-514 p.
Keyword [en]
Thioredoxin, Thioredoxin reductase, cis-Diamminedichloroplatinum (II), Cytotoxicity, Redox, Free radicals
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-98827PubMedID: 10490269OAI: oai:DiVA.org:liu-98827DiVA: diva2:655970
Available from: 2013-10-14 Created: 2013-10-14 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

No full text

Other links

PubMedLink to article

Authority records BETA

Spyrou, Giannis

Search in DiVA

By author/editor
Spyrou, Giannis
In the same journal
Free Radical Biology & Medicine
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

pubmed
urn-nbn

Altmetric score

pubmed
urn-nbn
Total: 24 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • oxford
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf