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Variations in Jun and Fos protein expression and AP-1 activity in cycling, resting and stimulated fibroblasts
Institut Pasteur, Paris cedex 15, France.
Novum, Karolinska Institute, Huddinge, Sweden.
Institut Pasteur, Paris cedex 15, France.
Institut Pasteur, Paris cedex 15, France.
1997 (English)In: Oncogene, ISSN 0950-9232, E-ISSN 1476-5594, Vol. 14, no 7, 819-830 p.Article in journal (Refereed) Published
Abstract [en]

We have analysed the different Jun and Fos proteins as NIH3T3 fibroblasts pass from exponential growth to quiescence and during the first 24 h after their re-entry into the cell cycle following serum stimulation. We show that these proteins can be divided into 3 subgroups based on their pattern of expression. The first contains c-Jun, Jun-D and Fra-2 which are expressed at high level in cycling cells and are only mildly induced by serum. The second contains Jun-B, c-Fos, Fos-B and deltaFos-B whose levels are low in cycling cells but increase strongly and rapidly after stimulation by serum. The third group contains only Fra-1, which is absent from cycling cells and behaves as a delayed early response protein after serum stimulation. AP-1 binding activity is low both in cycling and quiescent fibroblasts but increases after stimulation by serum with kinetics matching the induction of the various Jun and Fos proteins. Antibody supershift analyses demonstrate that the composition of AP-1 binding activity reflects the relative abundance of each Jun and Fos protein. Furthermore, the state of post-translational modification varies continuously for all of the AP-1 proteins as growth conditions change. These data indicate that AP-1 activity during the G0-G1 transition is finely regulated and complex, involving changes both in protein expression and in posttranslational modification.

Place, publisher, year, edition, pages
Nature Publishing Group, 1997. Vol. 14, no 7, 819-830 p.
Keyword [en]
Jun; Fos; AP-1; NIH3T3; cell cycle
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-98844DOI: 10.1038/sj.onc.1200901PubMedID: 9047389OAI: diva2:655992
Available from: 2013-10-14 Created: 2013-10-14 Last updated: 2013-10-22Bibliographically approved

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Spyrou, Giannis
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