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NK-lysin, a disulfide-containing effector peptide of T-lymphocytes, is reduced and inactivated by human thioredoxin reductase. Implication for a protective mechanism against NK-lysin cytotoxicity
Karolinska Institute, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden.
Karolinska Institute, Stockholm, Sweden and Karolinska Institute, Novum, Huddinge, Sweden.
1996 (English)In: Journal of Biological Chemistry, ISSN 0021-9258, E-ISSN 1083-351X, Vol. 271, no 17, 10116-10120 p.Article in journal (Refereed) Published
Abstract [en]

The cytotoxic and antibacterial polypeptide NK-lysin has a molecular mass of approximately 9 kDa and contains three disulfide bonds. The activity was highly dependent on intact disulfides, because the bactericidal effect on Escherichia coli and the cytolytic effect on human 3B6 lymphocytes was inhibited when NK-lysin was treated with dithiothreitol prior to incubation with the cells. NK-lysin was a direct substrate for human or calf thymus thioredoxin reductase and preincubation of the peptide with mammalian thioredoxin reductase, and NADPH abolished its antibacterial and cytolytic activities. The addition of human thioredoxin further enhanced the inhibitory effect of thioredoxin reductase and NADPH. In contrast, e. coli thioredoxin reductase showed no direct disulfide reductase activity with NK-lysin in agreement with previous data showing large differences in structure and substrate specificity between the mammalian and E. coli enzymes. NK-lysin is the first identified macromolecular disulfide substrate for human thioredoxin reductase apart from human thioredoxin. When 3B6 cells were incubated with NADPH, thioredoxin, and thioredoxin reductase prior to addition of NK-lysin, cytotoxicity was markedly reduced. These data suggest that thioredoxin reductase inactivates NK-lysin and provides a mechanism by which the cytotoxic activity of NK-lysin is regulated.

Place, publisher, year, edition, pages
American Society for Biochemistry and Molecular Biology, 1996. Vol. 271, no 17, 10116-10120 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:liu:diva-98847DOI: 10.1074/jbc.271.17.10116PubMedID: 8626570OAI: diva2:655997
Available from: 2013-10-14 Created: 2013-10-14 Last updated: 2013-10-22Bibliographically approved

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Spyrou, Giannis
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