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High-level expression of fully active human glutaredoxin (thioltransferase) in E. coli and characterization of Cys7 to Ser mutant protein
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden.
1996 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 378, no 1, 69-73 p.Article in journal (Refereed) Published
Abstract [en]

Glutaredoxin (Grx) (12 kDa) is a hydrogen donor for ribonucleotide reductase and also a general GSH-disulfide reductase of importance for redox regulation. To overexpress human glutaredoxin in Escherichia coli, a cDNA encoding human Grx was modified and cloned into the vector pET-3d and expressed in E. coli BL21 (DE3) by IPTG induction. High-level expression of Grx was verified by GSH-disulfide oxidoreductase activity, SDS-PAGE and immunoblotting analysis. The recombinant human Grx in its reduced form was purified to homogenity with 50% yield and exhibited the same dehydroascorbate reductase and hydrogen donor activity for ribonucleotide reductase (Km approximately 0.2 microM) as the human placenta protein. Human Grx contains a total of 5 half-cystine residues including a non-conserved Cys7 residue and is easily oxidized to form dimers during storage. A Grx mutant Cys7 to Ser was generated by site-directed mutagenesis and the protein was purified to homogeneity. The mutant protein showed full activity and exhibited a much reduced tendency to form dimers compared with the wild type protein. Peptide sequencing confirmed the mutation and removal of the N-terminal Met residue in both wild type and mutant proteins. Fluorescence spectra demonstrated only tyrosine fluorescence in human Grx with a peak at 310 nm which increased 20% upon reduction and decreased by addition of GSSG demonstrating that glutathione-containing disulfides are excellent substrates.

Place, publisher, year, edition, pages
Elsevier, 1996. Vol. 378, no 1, 69-73 p.
Keyword [en]
Glutaredoxin; Thioltransferase; Thiol-disulfide; Recombinant protein; Site-directed mutagenesis; Fluorescence
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:liu:diva-98851PubMedID: 8549805OAI: oai:DiVA.org:liu-98851DiVA: diva2:656003
Available from: 2013-10-14 Created: 2013-10-14 Last updated: 2017-12-06Bibliographically approved

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Spyrou, Giannis

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