Generation of normal T and B lymphocytes by c-jun deficient embryonic stem cells
1994 (English)In: Immunity, ISSN 1074-7613, E-ISSN 1097-4180, Vol. 1, no 1, 65-72 p.Article in journal (Refereed) Published
To determine the potential roles of c-jun in lymphocyte development, we generated somatic chimeric mice by injecting homozygous c-jun mutant embryonic stem (ES) cells into blastocysts from recombination activating gene-2 (RAG-2)-deficient mice. Chimeric mice had poor restoration of thymocytes, but contained substantial numbers of mature T and B lymphocytes in the periphery. Stimulation of c-jun-/- B cells resulted in normal levels of proliferation and immunoglobulin secretion. Likewise, stimulation of c-jun-/- T cells resulted in essentially normal levels of IL-2R alpha expression, IL-2 secretion, and proliferation. We further showed that the relatively normal activation responses of the c-jun-/- T cells probably results from the fact that other members of the Jun family contribute to the bulk of the activator protein-1 (AP-1) complexes in normal T cells and, as a result, AP-1 complexes are found at relatively normal levels in c-jun-/- T cells.
Place, publisher, year, edition, pages
Cell Press , 1994. Vol. 1, no 1, 65-72 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:liu:diva-98854PubMedID: 7889400OAI: oai:DiVA.org:liu-98854DiVA: diva2:656006